Immunomodulatory and behavioral effects of ayahuasca and N, N-dimethyltryptamine in a rat model of lipopolysaccharide-induced depression.

IF 3.5 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Daniel do Nascimento Sousa, Monique de Azevedo, Maria Lucília Santos, Tatiana Karla Dos Santos Borges, Daniela Mara de Oliveira, Eloisa Dutra Caldas
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Abstract

Ayahuasca (Aya) is an Amazonian beverage traditionally used as medicine by Indigenous people in South America to treat various illnesses and have shown a potential to treat depression. This study aimed to investigate the antidepressant effects of fluoxetine, Aya and N, N dimethyl tryptamine (DMT), a component of the beverage, focusing on the modulation of inflammatory serum cytokine profiles and behavior of Wistar rats subjected to lipopolysaccharide (LPS)-induced depression. In total, 126 rats were randomly assigned to seven groups: saline control, LPS, fluoxetine, three ayahuasca groups (dosed at 0.5, 1, and 2 times the usual ritualistic dose, Aya0.5, Aya1 and Aya2), and one DMT treatment group. The rats received LPS every other day from day 1 to 13 and fluoxetine, Aya and DMT daily from day 2 to 14. At day 15, the rats were submitted to open field and forced swimming tests, plasma samples were collected and the animals were euthanized. The LPS group showed lower body weight grain and higher plasma levels of pro-inflammatory cytokines IL-1α (p < 0.001), TNF-α, and IL-12p70 compared to control, which were significantly reduced by the treatment groups (p < 0.05 up to p < 0.0001), indicating a potential for modulation of the inflammatory state seen in depression. The Aya2 group exhibited increased locomotion in the open field arena compared to the fluoxetine (p < 0.05) and DMT (p < 0.01) groups, with a significantly higher percentage of entries into the center than the control group (p < 0.01). Furthermore, treatments with fluoxetine, Aya, and DMT significantly increased swimming time compared to the LPS group (p < 0.01), and fluoxetine and the Aya0.5 groups displayed higher climbing times compared to LPS and control (p < 0.05). Although the LPS model did not consistently induce depressive-like behaviors, the results highlight the potential of ayahuasca and DMT to modulate the immune system and reduce pro-inflammatory cytokine levels associated with depression, which could have significant implications for treating inflammation-related aspects of depression.

死藤水和N, N-二甲基色胺对脂多糖诱导抑郁大鼠模型的免疫调节和行为影响。
死藤水(Aya)是一种亚马逊地区的饮料,传统上被南美土著人用作药物来治疗各种疾病,并显示出治疗抑郁症的潜力。本研究旨在探讨氟西汀、Aya和饮料成分N, N二甲基色胺(DMT)的抗抑郁作用,重点研究脂多糖(LPS)诱导的Wistar大鼠炎症血清细胞因子谱和行为的调节。126只大鼠被随机分为7组:生理盐水对照组、LPS组、氟西汀组、3个死藤水组(剂量分别为常规仪式剂量的0.5、1和2倍,分别为Aya0.5、Aya1和Aya2)和1个DMT治疗组。第1 ~ 13天每隔一天给药LPS,第2 ~ 14天每天给药氟西汀、Aya和DMT。第15天,将大鼠置于野外和强迫游泳试验中,采集血浆样本并实施安乐死。LPS组大鼠体质量颗粒降低,血浆促炎因子IL-1α水平升高(p
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来源期刊
Metabolic brain disease
Metabolic brain disease 医学-内分泌学与代谢
CiteScore
5.90
自引率
5.60%
发文量
248
审稿时长
6-12 weeks
期刊介绍: Metabolic Brain Disease serves as a forum for the publication of outstanding basic and clinical papers on all metabolic brain disease, including both human and animal studies. The journal publishes papers on the fundamental pathogenesis of these disorders and on related experimental and clinical techniques and methodologies. Metabolic Brain Disease is directed to physicians, neuroscientists, internists, psychiatrists, neurologists, pathologists, and others involved in the research and treatment of a broad range of metabolic brain disorders.
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