EIF3B Promotes KRAS Gene Mutation-Driven Colon Adenocarcinoma Progression Through the TBK1 and PI3K/AKT Signaling Pathways

IF 3.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chongren Ren, Yaxin Qin, Dujuan Cao, Xiaojing Ren, Haoliang Zhao, Jianli Han, Gang Du
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引用次数: 0

Abstract

Therapeutic targets involved in multiple signaling pathways offer promising treatment options for cancers driven by KRAS gene mutations (KRASmut). EIF3B has emerged as a potential therapeutic target for colorectal cancer (CRC). To elucidate the role of EIF3B and its interactions with potential targets in CRC, we utilized bioinformatics techniques alongside clinicopathological analyses. RNA sequencing identified signaling pathways associated with EIF3B. The expression levels of relevant genes and their associated pathways were measured using RT-qPCR and western blot analyses. RNA immunoprecipitation (RIP) confirmed the interactions between EIF3B and these specific genes. Analysis of data from The Cancer Genome Atlas (TCGA), combined with clinicopathological studies, revealed significantly elevated levels of EIF3B and TBK1 mRNA and proteins in KRASmut colorectal adenocarcinoma (COAD) compared to normal tissues. Silencing EIF3B inhibited the proliferation and progression of KRASmut COAD and disrupted the TBK1, PI3K/AKT, and JAK2/STAT3 pathways. EIF3B also regulated the translation of TBK1 and PIK3CA. Targeting EIF3B presents a novel approach to modulating various cellular signaling pathways, offering a promising therapeutic strategy for patients with KRASmut COAD.

EIF3B通过TBK1和PI3K/AKT信号通路促进KRAS基因突变驱动的结肠癌进展
涉及多种信号通路的治疗靶点为KRAS基因突变(KRASmut)驱动的癌症提供了有希望的治疗选择。EIF3B已成为结直肠癌(CRC)的潜在治疗靶点。为了阐明EIF3B在结直肠癌中的作用及其与潜在靶点的相互作用,我们利用了生物信息学技术和临床病理分析。RNA测序鉴定了与EIF3B相关的信号通路。RT-qPCR和western blot检测相关基因及其通路的表达水平。RNA免疫沉淀(RIP)证实了EIF3B与这些特异性基因之间的相互作用。癌症基因组图谱(TCGA)数据分析结合临床病理研究显示,与正常组织相比,KRASmut结直肠腺癌(COAD)中EIF3B和TBK1 mRNA和蛋白水平显著升高。沉默EIF3B抑制KRASmut COAD的增殖和进展,并破坏TBK1、PI3K/AKT和JAK2/STAT3通路。EIF3B也调节TBK1和PIK3CA的翻译。靶向EIF3B提供了一种调节多种细胞信号通路的新方法,为KRASmut COAD患者提供了一种有希望的治疗策略。
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来源期刊
CiteScore
5.80
自引率
2.80%
发文量
277
审稿时长
6-12 weeks
期刊介绍: The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.
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