GluN2B-Derived Small Interfering Peptide (GluN2B-Sip) Protects Against Sevoflurane-Induced Neurotoxicity in Developing Rats

IF 4 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Neurochemistry Pub Date : 2025-06-12 DOI:10.1111/jnc.70118

Kun Zhang, Yafang Liu, Zhaoxia Liao, Mingfang Zhang, Jing Zhang, Yujuan Li

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引用次数: 0

Abstract

Sevoflurane is widely used in pediatric anesthesia due to its rapid induction, minimal airway irritation, and stable hemodynamics. However, concerns have been raised regarding its potential adverse effects on cognition. Recent studies suggest that GluN2B-containing NMDA receptors play a key role in the memory deficits associated with sevoflurane exposure. In this study, we investigated whether the GluN2B small interfering peptide (GluN2B-sip) could protect against neurotoxicity induced by neonatal sevoflurane exposure. Our findings show that neonatal sevoflurane exposure leads to an increase in apoptosis-related proteins, long-term memory impairment, and reduced levels of synaptic proteins in the hippocampus of adolescent rats. GluN2B-sip partially restored synapse development and cognitive function. Together, these results suggest that GluN2B-sip mitigates sevoflurane-induced neurodegeneration and cognitive impairment in developing rats, providing a potential therapeutic strategy to prevent sevoflurane neurotoxicity. The Cdk5/GluN2B and Cdk5/CRMP2 signaling pathways may play central roles in this protective effect.

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glun2b衍生的小干扰肽（GluN2B-Sip）对发育中的大鼠七氟醚诱导的神经毒性有保护作用

七氟醚因其诱导迅速、对气道刺激最小、血流动力学稳定等优点被广泛应用于小儿麻醉。然而，人们对其对认知的潜在不利影响表示担忧。最近的研究表明，含有glun2b的NMDA受体在七氟醚暴露相关的记忆缺陷中起关键作用。在这项研究中，我们研究了GluN2B小干扰肽（GluN2B-sip）是否对新生儿七氟醚暴露引起的神经毒性具有保护作用。我们的研究结果表明，新生儿七氟醚暴露导致青春期大鼠海马细胞凋亡相关蛋白增加、长期记忆障碍和突触蛋白水平降低。GluN2B-sip部分恢复突触发育和认知功能。总之，这些结果表明GluN2B-sip减轻了七氟醚诱导的大鼠神经变性和认知障碍，为预防七氟醚神经毒性提供了一种潜在的治疗策略。Cdk5/GluN2B和Cdk5/CRMP2信号通路可能在这种保护作用中发挥核心作用。

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来源期刊

Journal of Neurochemistry 医学-神经科学

CiteScore

9.30

自引率

2.10%

发文量

181

审稿时长

2.2 months

期刊介绍： Journal of Neurochemistry focuses on molecular, cellular and biochemical aspects of the nervous system, the pathogenesis of neurological disorders and the development of disease specific biomarkers. It is devoted to the prompt publication of original findings of the highest scientific priority and value that provide novel mechanistic insights, represent a clear advance over previous studies and have the potential to generate exciting future research.