Heavy Metals and Carbapenem-Resistant Klebsiella pneumoniae in a River System of Odisha, India: Correlation and Integrated Risk Assessment.

IF 3.3 3区 生物学 Q2 ECOLOGY
Pragyan Paramita Swain, Enketeswara Subudhi, Rajesh Kumar Sahoo
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Abstract

The unregulated release of heavy metals and antibiotics into rivers has the potential to significantly impact human health. Infections caused by healthcare-associated pathogen, carbapenem-resistant Klebsiella pneumoniae (CRKP), present a critical challenge to clinical practitioners due to its resistance to last-line antibiotics. In this study, we investigated co-contamination of heavy metals (As, Cd, Cr, Mn, and Pb) and CRKP isolates in water samples from multiple sites along the river receiving wastewater discharge from urban areas of twin-city, Odisha. We used a composite risk scoring framework integrating chemical risks (based on hazard indices (HI) of heavy metals) and biological risks (based on the proportion of CRKP isolates exhibiting multidrug-resistant phenotypes and their multiple antibiotic resistance (MAR) index. Furthermore, Spearman's correlations and redundancy analysis (RDA) were employed to assess the association between heavy metals and antibiotic resistance genes (ARGs). From the total CRKP isolates identified (n = 91), 90.1% and 9.89% exhibited multidrug resistant (MDR) and extensively drug-resistant (XDR) phenotypes, respectively. Sites D2 and C2 were flagged as high-risk sites based on their composite risk scores of 0.735 and 0.699, respectively. Positive correlations were observed between heavy metals and ARGs (blaOXA-48, blaTEM, and blaSHV). The findings raise concern regarding the potential threat of CRKP and heavy metal pollution in river water while also emphasizing the need for integrated assessment to control their release into the environment.

印度奥里萨邦河系重金属与耐碳青霉烯肺炎克雷伯菌:相关性与综合风险评估。
重金属和抗生素不受管制地排放到河流中有可能对人类健康产生重大影响。由卫生保健相关病原体引起的感染,碳青霉烯耐药肺炎克雷伯菌(CRKP),由于其对最后一线抗生素的耐药性,对临床医生提出了严峻的挑战。在这项研究中,我们调查了来自奥里萨邦双城城市地区污水排放河流沿线多个站点的水样中重金属(As, Cd, Cr, Mn和Pb)和CRKP分离物的共同污染。我们使用了一个综合风险评分框架,将化学风险(基于重金属的危害指数(HI))和生物风险(基于显示多重耐药表型的CRKP分离株的比例及其多重抗生素耐药性(MAR)指数)整合在一起。此外,采用Spearman相关和冗余分析(RDA)来评估重金属与抗生素耐药基因(ARGs)之间的关系。在所鉴定的CRKP分离株(n = 91)中,90.1%和9.89%分别表现出多重耐药(MDR)和广泛耐药(XDR)表型。D2和C2的综合风险评分分别为0.735和0.699,被标记为高风险站点。重金属与ARGs (blaOXA-48、blaTEM和blaSHV)呈正相关。研究结果引起了人们对CRKP和河水重金属污染的潜在威胁的关注,同时也强调需要进行综合评估,以控制它们向环境中的释放。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbial Ecology
Microbial Ecology 生物-海洋与淡水生物学
CiteScore
6.90
自引率
2.80%
发文量
212
审稿时长
3-8 weeks
期刊介绍: The journal Microbial Ecology was founded more than 50 years ago by Dr. Ralph Mitchell, Gordon McKay Professor of Applied Biology at Harvard University in Cambridge, MA. The journal has evolved to become a premier location for the presentation of manuscripts that represent advances in the field of microbial ecology. The journal has become a dedicated international forum for the presentation of high-quality scientific investigations of how microorganisms interact with their environment, with each other and with their hosts. Microbial Ecology offers articles of original research in full paper and note formats, as well as brief reviews and topical position papers.
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