Advancing Protein-Coding RNA Engineering: From Structural Refinement to Biomedical Implementation

IF 3.1 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Xuan Lu, Shuangpeng Li, Yue Chi, Shuo Lin, Hui Yuan, Zhiyi Lin, Yingying Xiao, Haihua Yin, Xia Zuo, Ruilin Cheng, Jiahui Xi, Min Chen, Qingjian Zou
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引用次数: 0

Abstract

Messenger RNA (mRNA), emerging as a revolutionary therapeutic tool, has shown remarkable potential in diverse fields such as vaccine development, tumor immunotherapy, gene therapy, and protein replacement therapy, attributed to its high programmability and safety. However, traditional engineered mRNA therapeutics encounter obstacles like short half-lives and limited protein expression, which impede their extensive application in the biomedical domain. In recent years, with the progress of RNA modification technologies and the advent of novel RNA techniques, including mRNA, self-amplifying RNA (saRNA), circular RNA (circRNA), and branched RNA (brRNA), researchers have achieved substantial breakthroughs in enhancing RNA stability, prolonging protein expression, and reducing immunogenicity. This article comprehensively reviews the structure, function, optimization strategies, and biomedical applications of these protein-coding RNAs.

Abstract Image

推进蛋白质编码RNA工程:从结构改进到生物医学实施
信使RNA (mRNA)作为一种革命性的治疗工具,由于其高度可编程性和安全性,在疫苗开发、肿瘤免疫治疗、基因治疗、蛋白质替代治疗等多个领域显示出巨大的潜力。然而,传统的工程化mRNA治疗方法遇到半衰期短和蛋白质表达有限等障碍,阻碍了它们在生物医学领域的广泛应用。近年来,随着RNA修饰技术的进步和mRNA、自扩增RNA (saRNA)、环状RNA (circRNA)、支链RNA (brRNA)等新型RNA技术的出现,研究人员在增强RNA稳定性、延长蛋白表达、降低免疫原性等方面取得了重大突破。本文综述了这些蛋白质编码rna的结构、功能、优化策略及其在生物医学上的应用。
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来源期刊
Biotechnology Journal
Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
8.90
自引率
2.10%
发文量
123
审稿时长
1.5 months
期刊介绍: Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.
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