Role of the CEACAM7-JAK2/STAT3-BST2 Axis in the migration of nasopharyngeal carcinoma cells

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular basis of disease Pub Date : 2025-06-05 DOI:10.1016/j.bbadis.2025.167948

Ju-Pi Li , Chiao-Wen Lin , Yen-Ting Lu , Cheng-Chen Huang , Yu-Ting Ho , Chung-Han Hsin , Shun-Fa Yang

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引用次数: 0

Abstract

Objective

Nasopharyngeal carcinoma (NPC) is a rare malignancy of the head and neck, with recurrence and distant metastasis being the primary causes of mortality among affected patients. Carcinoembryonic antigen cellular adhesion molecules (CEACAMs) are implicated in various physiological and pathophysiological processes. However, the role of CEACAM7 in NPC progression remains unclear. This study investigated the influence of CEACAM7 on NPC progression.

Methods

Cell migration and invasion ability were conducted using a Boyden chamber assay. Regulation of signaling pathways was evaluated by immunoblotting.

Results

The findings revealed that CEACAM7 is highly expressed in human NPC tissues, with particularly elevated levels observed in malignant tissues and high-grade squamous cell carcinoma tissues. Furthermore, CEACAM7 was demonstrated to regulate cell migration and invasion in human NPC cells but did not influence cell proliferation. Moreover, CEACAM7 enhanced cell motility through the activation of the JAK2/STAT3/BST2 signaling pathway. Notably, cotreatment with WP1066, a JAK2/STAT3 inhibitor, suppressed CEACAM7-induced cell migratory ability and downregulated BST2 expression in NPC cells.

Conclusions

These findings indicate that the CEACAM7/JAK2/STAT3/BST2 axis plays a crucial role in promoting NPC cell migration and underscore its potential as a therapeutic target for mitigating NPC metastasis.

Abstract Image

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CEACAM7-JAK2/STAT3-BST2轴在鼻咽癌细胞迁移中的作用

目的鼻咽癌（NPC）是一种罕见的头颈部恶性肿瘤，复发和远处转移是患者死亡的主要原因。癌胚抗原细胞粘附分子（CEACAMs）参与多种生理和病理生理过程。然而，CEACAM7在NPC进展中的作用尚不清楚。本研究探讨了CEACAM7对鼻咽癌进展的影响。方法采用Boyden室法测定细胞迁移和侵袭能力。通过免疫印迹法评估信号通路的调节。结果CEACAM7在人鼻咽癌组织中高表达，在恶性组织和高级别鳞状细胞癌组织中高表达。此外，CEACAM7被证明可以调节人鼻咽癌细胞的细胞迁移和侵袭，但不影响细胞增殖。此外，CEACAM7通过激活JAK2/STAT3/BST2信号通路增强细胞运动性。值得注意的是，与JAK2/STAT3抑制剂WP1066共处理，抑制了ceacam7诱导的细胞迁移能力，下调了鼻咽癌细胞中BST2的表达。结论CEACAM7/JAK2/STAT3/BST2轴在促进鼻咽癌细胞迁移中起重要作用，并提示其可能作为缓解鼻咽癌转移的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochimica et biophysica acta. Molecular basis of disease 生物-生化与分子生物学

CiteScore

12.30

自引率

0.00%

发文量

218

审稿时长

32 days

期刊介绍： BBA Molecular Basis of Disease addresses the biochemistry and molecular genetics of disease processes and models of human disease. This journal covers aspects of aging, cancer, metabolic-, neurological-, and immunological-based disease. Manuscripts focused on using animal models to elucidate biochemical and mechanistic insight in each of these conditions, are particularly encouraged. Manuscripts should emphasize the underlying mechanisms of disease pathways and provide novel contributions to the understanding and/or treatment of these disorders. Highly descriptive and method development submissions may be declined without full review. The submission of uninvited reviews to BBA - Molecular Basis of Disease is strongly discouraged, and any such uninvited review should be accompanied by a coverletter outlining the compelling reasons why the review should be considered.