Early diagnosis value of lncRNA SNHG14 combined with miR-493-5p in gestational diabetes mellitus and its correlation with pregnancy outcome.

Abstract

BackgroundTo evaluate the diagnostic value of lncRNA SNHG14 and miR-493-5p in gestational diabetes mellitus (GDM).MethodsThis study consisted of 128 GDM patients and 125 healthy controls. Dual luciferase reporter assay detected binding of lncRNA SNHG14 and miR-493-5p. ROC was applied to evaluate the diagnostic value of lncRNA SNHG14 and miR-493-5p for GDM. Independent factors affecting GDM were evaluated using logistic regression analysis. The role of lncRNA SNHG14, miR-493-5p in pregnancy outcome in GDM patients was assessed by chi-square tests.ResultsLncRNA SNHG14 has a target-binding relationship with miR-493-5p. LncRNA SNHG14 was significantly higher in GDM patients, while miR-493-5p was significantly lower; both were independent risk factors for GDM development. Blood glucose indices (fasting, 1 h and 2 h postprandial) were positively correlated with lncRNA SNHG14 and negatively correlated with miR-493-5p in GDM patients. LncRNA SNHG14 in combination with miR-493-5p increased the diagnostic efficiency for GDM. LncRNA SNHG14 was significantly associated with neonatal weight and neonatal jaundice. MiR-493-5p was significantly associated with neonatal jaundice.ConclusionLncRNA SNHG14 in combination with miR-493-5p may be an early diagnostic marker of GDM. LncRNA SNHG14 and miR-493-5p are associated with adverse pregnancy outcomes (macrosomia, neonatal jaundice) in patients with GDM.