The effects of prepubertal ovariectomy and chronic stress on activity of brain limbic regions in adult mice.

IF 2.6 4区 心理学 Q2 BEHAVIORAL SCIENCES
Emma Woodward, Sydney Fitzcharles, Laurence Coutellier
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引用次数: 0

Abstract

Women are twice as likely than men to develop an anxiety disorder after stress exposure stress, a sex-specific vulnerability that arises after puberty. This suggests that pubertal hormones could contribute to the central changes induced by stress and leading to behavioral deregulations. The main brain regions involved in stress-induced psychopathologies are part of the limbic system, including the prefrontal cortex (PFC), the basolateral amygdala (BLA) and the ventral hippocampus (vHipp). Changes in their activity are often reported after stress, contributing to appearance of behavioral symptoms. We aimed at determining whether female pubertal hormones modulate the effects of chronic stress on activity of these limbic regions to identify a potential mechanism underlying the female vulnerability to stress-induced pathologies. Prepubertal adolescent female mice underwent ovariectomy (OVX) or sham surgery. In late adolescence, they started 4 weeks of unpredictable chronic mild stress (UCMS). We used immunohistochemistry to quantify FosB/ΔFosB, a marker of chronic activity, in the PFC, BLA, and vHipp. Prepubertal OVX increased activity of the PFC and decreased activity of the BLA and vHipp, while UCMS had little impact. The PFC was more significantly impacted by both OVX and UCMS, with each of these manipulations increasing number of FosB/ΔFosB+ cells, however without interactive effects. Correlation analyses indicate that level of activity in the PFC and vHipp correlates with measures of anxiety. However, the gonadal status influences strongly these relationships. Our data indicate that pubertal hormones could play a role in the regulation of anxiety through their long-lasting impact on the limbic system.

青春期前卵巢切除和慢性应激对成年小鼠脑边缘区活动的影响。
女性在压力暴露后患上焦虑症的可能性是男性的两倍,这是青春期后出现的一种性别特异性脆弱性。这表明青春期激素可能有助于压力引起的中枢变化,并导致行为失调。与压力引起的精神病理有关的主要大脑区域是边缘系统的一部分,包括前额叶皮层(PFC)、基底外侧杏仁核(BLA)和腹侧海马体(vHipp)。他们的活动变化通常在压力后报告,导致行为症状的出现。我们旨在确定女性青春期激素是否调节慢性应激对这些边缘区域活动的影响,以确定女性易受应激诱导病理影响的潜在机制。接受卵巢切除术(OVX)或假手术的青春期前雌性小鼠。在青春期后期,他们开始了为期4周的不可预测的慢性轻度压力(UCMS)。我们使用免疫组织化学方法量化了PFC、BLA和vHipp中慢性活性标记物FosB/ΔFosB。青春期前OVX增加了PFC活性,降低了BLA和vHipp活性,而UCMS对PFC活性的影响不大。OVX和UCMS对PFC的影响更为显著,每种操作都增加了FosB/ΔFosB+细胞的数量,但没有相互作用。相关分析表明,PFC和vHipp的活动水平与焦虑程度相关。然而,性腺状态强烈地影响着这些关系。我们的数据表明,青春期激素可能通过对大脑边缘系统的长期影响,在调节焦虑方面发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.60
自引率
0.00%
发文量
25
审稿时长
6-12 weeks
期刊介绍: The journal Stress aims to provide scientists involved in stress research with the possibility of reading a more integrated view of the field. Peer reviewed papers, invited reviews and short communications will deal with interdisciplinary aspects of stress in terms of: the mechanisms of stressful stimulation, including within and between individuals; the physiological and behavioural responses to stress, and their regulation, in both the short and long term; adaptive mechanisms, coping strategies and the pathological consequences of stress. Stress will publish the latest developments in physiology, neurobiology, molecular biology, genetics research, immunology, and behavioural studies as they impact on the understanding of stress and its adverse consequences and their amelioration. Specific approaches may include transgenic/knockout animals, developmental/programming studies, electrophysiology, histochemistry, neurochemistry, neuropharmacology, neuroanatomy, neuroimaging, endocrinology, autonomic physiology, immunology, chronic pain, ethological and other behavioural studies and clinical measures.
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