Extended time to maturity in Anopheles coluzzii: Implications of late egg hatch for vector control and transgene fitness.

Abstract

Maintaining fitness is an important consideration when mosquitoes are mass-reared for the deployment of genetic interventions that are designed to suppress populations because released mosquitoes need to compete with wild-type mosquitoes. Late-hatching mosquitoes are more suitable for transportation to remote field sites. Here, we investigated the fitness of late-hatching phenotypes in Anopheles coluzzii. Selected lines of the VK strain (from Burkina Faso) were created through bidirectional selection for early and late hatching, over 20 generations. These were compared with each other and the established Mopti reference strain from Mali, reared in the lab for >16 years. Significant differences in life-history traits were found between Mopti and VK strains but few differences were found between the selected VK lines. Considering that late-hatching VK lines showed no evidence of fitness costs, our results suggest that the late selected VK lines, which start hatching after 4 days, are an alternative option for egg shipment for mass mosquito releases over the well-established Mopti that hatches within 2 days and has lower adult survival.