Extraction and Activity Mechanism of α-Glucosidase Inhibitory Peptides From Seabuckthorn Seed Meal

IF 3.4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2025-06-06 DOI:10.1111/1750-3841.70269

Wenwen Li, Tingting Xu, Yanan Yuan, Shuxin Tang, Ting Wu, Siyi Pan, Xiaoyun Xu

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引用次数: 0

Abstract

ABSTRACT

Seabuckthorn seed meal (SSM) is a by-product of the oil extraction process from seabuckthorn seeds. Despite its high protein content, SSM remains largely underutilized. This study aimed to extract seabuckthorn seed meal protein peptides (SSPPs) with hypoglycemic activity and investigate their mechanism of action. The optimized conditions yielded a peptide extraction rate of 20.99% ± 1.09%, achieved through ultrasound pretreatment for 49 min, the addition of 10% alkaline protease, an initial pH of 11, and a 2-h enzymatic hydrolysis time. Notably, the half inhibitory concentration (IC₅₀) against α-glucosidase was determined to be 0.18 ± 0.02 mg/mL, which is superior to that of seabuckthorn seed meal protein (SSP) and acarbose, and demonstrated stability under various processing conditions. Structural comparisons between SSP and SSPP indicated that a higher proportion of random coils in the secondary structure, irregularities in surface morphology, and increased surface hydrophobicity contributed to α-glucosidase inhibition. Inhibitory kinetic analysis indicated that SSPP exerted a direct effect on α-glucosidase activity. Furthermore, four novel α-glucosidase inhibitory peptides were identified through ultrafiltration-affinity-liquid-mass spectrometry, all of which compete with substrates for the enzyme's active site, namely, SER-466 and LYS-665. A significant positive correlation was observed between the molecular docking results and the inhibition outcomes of the synthesized peptides (r = 0.895, p < 0.05). In conclusion, this study supports the potential of SSPP as a natural hypoglycemic food additive that can significantly enhance the value of seabuckthorn resources.

Practical Application: The SSPP developed in this research demonstrated a significant α-glucosidase inhibition rate and exhibited commendable activity stability, thereby offering both technical and theoretical foundations for the high-value application of seabuckthorn seed meal.

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沙棘籽粕中α-葡萄糖苷酶抑制肽的提取及活性机制研究

沙棘籽粕（SSM）是沙棘籽榨油过程中的副产品。尽管其蛋白质含量高，但SSM在很大程度上仍未得到充分利用。本研究旨在提取具有降血糖活性的沙棘籽粕蛋白肽，并探讨其作用机制。优化条件为超声预处理49 min，添加10%碱性蛋白酶，初始pH为11，酶解时间为2 h，肽提取率为20.99%±1.09%。值得注意的是，其对α-葡萄糖苷酶的半抑制浓度（IC50）为0.18±0.02 mg/mL，优于沙棘籽粕蛋白（SSP）和阿卡波糖，且在各种加工条件下均表现出稳定性。SSP和SSPP的结构比较表明，SSP的二级结构中随机线圈的比例更高，表面形态不规则，表面疏水性增强，这是α-葡萄糖苷酶抑制的原因。抑制动力学分析表明，SSPP对α-葡萄糖苷酶活性有直接影响。此外，通过超滤-亲和-液相质谱法鉴定了四种新的α-葡萄糖苷酶抑制肽，它们都与酶的活性位点竞争，即SER-466和LYS-665。分子对接结果与合成肽的抑制效果呈显著正相关(r = 0.895, p <；0.05)。综上所述，本研究支持SSPP作为一种天然降糖食品添加剂的潜力，可以显著提高沙棘资源的价值。实际应用：本研究制备的SSPP具有显著的α-葡萄糖苷酶抑制率和良好的活性稳定性，为沙棘籽粕的高价值应用提供了技术和理论基础。

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.