How to Harvest and Store Corn (Zea mays).

Miriam Nancy Salazar-Vidal, Melissa A Draves, Sarah L Fitzsimmons, Zachary B Traylor, Sherry Flint-Garcia
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引用次数: 0

Abstract

Harvest scheduling, seed drying, and good storage practices are essential for maize research to avoid negative impacts on the quality of the seeds and to maximize seed viability. Embryo growth and accumulation of energy reserves in the endosperm are completed ∼40 days after pollination, of which the last 10-20 days are devoted to maturation and desiccation. Seed maturity is affected by many factors including temperature, day length, humidity, and soil moisture. Once seeds are mature, they must be harvested. Hand harvesting, which allows for greater control and minimizes ear damage, is primarily used in genetic nurseries and general research because each genotype is represented by a small number of plants. Hand harvesting is also used where there is a mix of manually pollinated and undesired open pollinated ears, and can be a selective harvest depending on the research objectives. However, hand harvesting is more labor-intensive and time-consuming compared to combine harvesting. If harvesting a yield trial, where the purpose is to collect yield data and identify promising genotypes, the use of a combine (not described in this protocol) is critical to consistently capture grain weight, moisture, and test weight for each plot. Following harvest, materials must be dried to the appropriate moisture content before storage. Corn is typically stored using the "active collection" model, with temperatures set between 5°C and 10°C and low relative humidity. This harvest protocol is intended to assist laboratories that are new to maize research and may be modified based on project goals, genetic material, equipment, or available space.

如何收获和储存玉米(玉米)。
为了避免对种子质量的负面影响,并最大限度地提高种子的活力,收获计划、种子干燥和良好的储存实践对玉米研究至关重要。胚的生长和胚乳能量储备的积累在授粉后约40天完成,其中最后10-20天用于成熟和干燥。影响种子成熟的因素有很多,包括温度、日照长度、湿度和土壤湿度。种子一旦成熟,就必须收割。手工收割可以更好地控制和最大限度地减少对耳朵的伤害,主要用于遗传苗圃和一般研究,因为每种基因型都由少数植物代表。手工收获也用于人工授粉和不希望开放授粉的耳朵混合的地方,并且可以根据研究目标进行选择性收获。然而,与联合收割机相比,手工采收更加劳动密集和耗时。如果进行产量试验,其目的是收集产量数据并确定有希望的基因型,则使用联合收割机(未在本方案中描述)对于持续捕获每个地块的粒重、水分和试验重量至关重要。收获后,物料在储存前必须干燥到适当的水分含量。玉米通常采用“主动收集”模式储存,温度设定在5°C至10°C之间,相对湿度较低。本收获方案旨在帮助新从事玉米研究的实验室,并可根据项目目标、遗传物质、设备或可用空间进行修改。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cold Spring Harbor protocols
Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.00
自引率
0.00%
发文量
163
期刊介绍: Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.
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