Registration of two peanut mutants TifGP-8 and TifGP-9 with reduced expression of peanut allergens Ara h 1.02 and Ara h 2.02

Abstract

Peanut (Arachis hypogaea L.) is a nutritious food source; however, it can cause serious allergic response among sensitized children and adults. Sixteen peanut proteins have been identified as allergens. Among those, vicilin protein Ara h 1 and conglutinin Ara h 2 are considered major peanut allergens due to the high frequency of recognition by IgE from allergic patients. Reducing the accumulation of Ara h 1 and Ara h 2 in peanut could potentially alleviate the severity of peanut allergic reactions. In order to identify allergen reduced lines, an ethyl methane sulfonate mutagenized peanut population was screened with gene specific primers by Targeting Induced Local Lesions in Genomes (TILLING) approach. TifGP-8 (Reg. no. GS-13, PI 701083) is a mutant line carrying a transitional C to T mutation in Ara h 1.02 which resulted in a premature stop codon and truncation in protein synthesis. TifGP-9 (Reg. no. GS-14, PI 701084) carries a G to A transition at the start codon of Ara h 2.02 resulting in a knockout of protein expression. Two cleaved amplified polymorphic sequence markers targeted at the mutation loci were developed to identify homozygous lines among progenies. Release of these allergen mutants will provide valuable genetic stocks for breeding reduced allergen peanuts.