OMIP-115: High-Dimensional Phenotypic Characterization of Human Natural Killer Cells for Therapeutic Use

IF 2.1 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Cytometry Part A Pub Date : 2025-06-02 DOI:10.1002/cyto.a.24941

Emil Birch Christensen, Moritz Schaefer, Mike Bogetofte Barnkob, Christian Nielsen, Torben Barington

引用次数: 0

Abstract

This 29-color flow cytometry panel was developed and optimized for in-depth characterization of human peripheral blood NK cells for preclinical development and monitoring of NK cell therapies. The panel includes markers associated with NK cell differentiation, cytotoxicity, tissue residency, as well as NK cell dysfunction. Panel optimization was performed on freshly isolated and ex vivo activated NK cells enriched from human peripheral blood mononuclear cells (PBMCs). Overall, this panel functions as a tool to extensively characterize human NK cells, paving the way for rapid and standardized approaches to evaluate the biological activity of therapeutic NK cell products.

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OMIP-115：用于治疗用途的人类自然杀伤细胞的高维表型表征。

这种29色流式细胞仪面板是为深入表征人类外周血NK细胞而开发和优化的，用于临床前开发和监测NK细胞疗法。该面板包括与NK细胞分化、细胞毒性、组织驻留以及NK细胞功能障碍相关的标记物。对从人外周血单核细胞（PBMCs）中富集的新鲜分离和离体活化NK细胞进行了面板优化。总的来说，该小组作为广泛表征人类NK细胞的工具，为快速和标准化的方法来评估治疗性NK细胞产品的生物活性铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytometry Part A 生物-生化研究方法

CiteScore

8.10

自引率

13.50%

发文量

183

审稿时长

4-8 weeks

期刊介绍： Cytometry Part A, the journal of quantitative single-cell analysis, features original research reports and reviews of innovative scientific studies employing quantitative single-cell measurement, separation, manipulation, and modeling techniques, as well as original articles on mechanisms of molecular and cellular functions obtained by cytometry techniques. The journal welcomes submissions from multiple research fields that fully embrace the study of the cytome: Biomedical Instrumentation Engineering Biophotonics Bioinformatics Cell Biology Computational Biology Data Science Immunology Parasitology Microbiology Neuroscience Cancer Stem Cells Tissue Regeneration.