Pushing the Boundaries of Resolution in Solid-State Nuclear Magnetic Resonance of Biomolecules with 160 kHz Magic-Angle Spinning

IF 15.6 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Journal of the American Chemical Society Pub Date : 2025-06-02 DOI:10.1021/jacs.5c02466

Zhiyu Sun, Claire Ollier, Adrienn Rancz, Batiste Thienpont, Kristof Grohe, Lukas Becker, Armin Purea, Frank Engelke, Sebastian Wegner, James Sturgis, Tatyana Polenova, Tanguy Le Marchand, Guido Pintacuda

引用次数: 0

Abstract

We present ultrafast magic-angle spinning (MAS) at 160 kHz in solid-state nuclear magnetic resonance (NMR), demonstrating unprecedented spectral quality and coherence lifetimes in proton-detected experiments on biomolecular systems. By optimizing experimental and sample conditions and utilizing advanced filling and handling tools, we achieve superior resolution on both microcrystalline and membrane-reconstituted proteins, paving the way for new applications in structural biology.

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用160千赫魔角旋转推动生物分子固态核磁共振分辨率的极限

我们在固态核磁共振（NMR）中提出了160 kHz的超快魔角旋转（MAS），在生物分子系统的质子检测实验中展示了前所未有的光谱质量和相干寿命。通过优化实验和样品条件以及利用先进的填充和处理工具，我们在微晶和膜重构蛋白上实现了卓越的分辨率，为结构生物学的新应用铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the American Chemical Society 化学-化学综合

CiteScore

24.40

自引率

6.00%

发文量

2398

审稿时长

1.6 months

期刊介绍： The flagship journal of the American Chemical Society, known as the Journal of the American Chemical Society (JACS), has been a prestigious publication since its establishment in 1879. It holds a preeminent position in the field of chemistry and related interdisciplinary sciences. JACS is committed to disseminating cutting-edge research papers, covering a wide range of topics, and encompasses approximately 19,000 pages of Articles, Communications, and Perspectives annually. With a weekly publication frequency, JACS plays a vital role in advancing the field of chemistry by providing essential research.