Microbial inoculants modulate the rhizosphere microbiome, alleviate plant stress responses, and enhance maize growth at field scale

IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genome Biology Pub Date : 2025-06-01 DOI:10.1186/s13059-025-03621-7

Davide Francioli, Ioannis D. Kampouris, Theresa Kuhl-Nagel, Doreen Babin, Loreen Sommermann, Jan H. Behr, Soumitra Paul Chowdhury, Rita Zrenner, Narges Moradtalab, Michael Schloter, Joerg Geistlinger, Uwe Ludewig, Günter Neumann, Kornelia Smalla, Rita Grosch

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引用次数: 0

Abstract

Field inoculation of crops with beneficial microbes is a promising sustainable strategy to enhance plant fitness and nutrient acquisition. However, effectiveness can vary due to environmental factors, microbial competition, and methodological challenges, while their precise modes of action remain uncertain. This underscores the need for further research to optimize inoculation strategies for consistent agricultural benefits. Using a comprehensive, multidisciplinary approach, we investigate the effects of a consortium of beneficial microbes (BMc) (Pseudomonas sp. RU47, Bacillus atrophaeus ABi03, Trichoderma harzianum OMG16) on maize (Zea mays cv. Benedictio) through an inoculation experiment conducted within a long-term field trial across intensive and extensive farming practices. Additionally, an unexpected early drought stress emerged as a climatic variable, offering further insight into the effectiveness of the microbial consortium. Our findings demonstrate that BMc root inoculation primarily enhanced plant growth and fitness, particularly by increasing iron uptake, which is crucial for drought adaptation. Inoculated maize plants show improved shoot growth and fitness compared to non-inoculated plants, regardless of farming practices. Specifically, BMc modulate plant hormonal balance, enhance the detoxification of reactive oxygen species, and increase root exudation of iron-chelating metabolites. Amplicon sequencing reveals shifts in rhizosphere bacterial and fungal communities mediated by the consortium. Metagenomic shotgun sequencing indicates enrichment of genes related to antimicrobial lipopeptides and siderophores. Our findings highlight the multifaceted benefits of BMc inoculation on plant fitness, significantly influencing metabolism, stress responses, and the rhizosphere microbiome. These improvements are crucial for advancing sustainable agricultural practices by enhancing plant resilience and productivity.

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微生物接种剂调节根际微生物群，缓解植物胁迫反应，促进玉米田间生长

作物田间接种有益微生物是一种很有前途的可持续策略，以提高植物的适应性和营养获取。然而，由于环境因素、微生物竞争和方法挑战，其有效性可能会有所不同，而其确切的作用模式仍不确定。这强调需要进一步研究以优化接种策略以获得一致的农业效益。采用综合的、多学科的方法，我们研究了一组有益微生物（BMc）（假单胞菌sp. RU47、萎缩芽孢杆菌ABi03、哈茨木霉OMG16）对玉米(Zea mays cv。通过在集约化和粗放化耕作方式的长期田间试验中进行的接种试验。此外，意外的早期干旱胁迫作为气候变量出现，为微生物联盟的有效性提供了进一步的见解。我们的研究结果表明，BMc根接种主要提高了植物的生长和适应性，特别是通过增加铁的吸收，这对干旱适应至关重要。无论耕作方式如何，接种玉米植株比未接种的植株表现出更好的芽部生长和适宜性。具体来说，BMc可以调节植物的激素平衡，增强活性氧的解毒能力，增加铁螯合代谢产物的根分泌。扩增子测序揭示了由该联盟介导的根际细菌和真菌群落的变化。宏基因组霰弹枪测序显示，与抗菌脂肽和铁载体相关的基因富集。我们的研究结果强调了BMc接种对植物适应性的多方面益处，显著影响代谢、胁迫反应和根际微生物组。这些改进对于通过提高植物抗灾能力和生产力来推进可持续农业实践至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genome Biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

21.00

自引率

3.30%

发文量

241

审稿时长

2 months

期刊介绍： Genome Biology stands as a premier platform for exceptional research across all domains of biology and biomedicine, explored through a genomic and post-genomic lens. With an impressive impact factor of 12.3 (2022),* the journal secures its position as the 3rd-ranked research journal in the Genetics and Heredity category and the 2nd-ranked research journal in the Biotechnology and Applied Microbiology category by Thomson Reuters. Notably, Genome Biology holds the distinction of being the highest-ranked open-access journal in this category. Our dedicated team of highly trained in-house Editors collaborates closely with our esteemed Editorial Board of international experts, ensuring the journal remains on the forefront of scientific advances and community standards. Regular engagement with researchers at conferences and institute visits underscores our commitment to staying abreast of the latest developments in the field.