Recent Advances in Mass Spectrometry-Based Studies of Post-translational Modifications in Alzheimer's Disease.

IF 6.1 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Molecular & Cellular Proteomics Pub Date : 2025-05-29 DOI:10.1016/j.mcpro.2025.101003

Feixuan Wu, Wei Li, Haiyan Lu, Lingjun Li

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引用次数: 0

Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline. There are over 10 million new cases of AD each year worldwide, implying one new case every 3.2 seconds. Post-translational modifications (PTMs) such as phosphorylation, glycosylation, and citrullination have emerged as key modulators of protein function in AD, influencing protein aggregation, clearance, and toxicity. Mass spectrometry (MS) has become an indispensable tool for detecting and quantifying these PTMs, offering valuable insights into their role in AD pathogenesis. This review explores recent advancements in MS-based studies of PTMs in AD, with emphasis on MS techniques like data-dependent acquisition (DDA) and data-independent acquisition (DIA), as well as enrichment methods used to characterize PTMs. The applications of these MS-based approaches to the study of various PTMs are highlighted, which have significantly accelerated the biomarker discovery process, providing new avenues for early diagnosis and therapeutic targeting. Advances in biological understanding and analytical techniques, while addressing the challenges and future directions, will be discussed.

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基于质谱的阿尔茨海默病翻译后修饰研究的最新进展。

阿尔茨海默病（AD）是一种以认知能力下降为特征的进行性神经退行性疾病。全世界每年有超过1000万新发AD病例，这意味着每3.2秒就有一例新发病例。翻译后修饰（PTMs）如磷酸化、糖基化和瓜氨酸化已成为AD中蛋白质功能的关键调节剂，影响蛋白质聚集、清除和毒性。质谱（MS）已成为检测和量化这些ptm的不可或缺的工具，为其在AD发病机制中的作用提供了有价值的见解。本文综述了以MS为基础的AD中PTMs研究的最新进展，重点介绍了数据依赖采集（DDA）和数据独立采集（DIA）等MS技术，以及用于表征PTMs的富集方法。重点介绍了这些基于ms的方法在各种ptm研究中的应用，这些方法显著加快了生物标志物的发现过程，为早期诊断和治疗靶向提供了新的途径。在解决挑战和未来方向的同时，将讨论生物理解和分析技术的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular & Cellular Proteomics 生物-生化研究方法

CiteScore

11.50

自引率

4.30%

发文量

131

审稿时长

84 days

期刊介绍： The mission of MCP is to foster the development and applications of proteomics in both basic and translational research. MCP will publish manuscripts that report significant new biological or clinical discoveries underpinned by proteomic observations across all kingdoms of life. Manuscripts must define the biological roles played by the proteins investigated or their mechanisms of action. The journal also emphasizes articles that describe innovative new computational methods and technological advancements that will enable future discoveries. Manuscripts describing such approaches do not have to include a solution to a biological problem, but must demonstrate that the technology works as described, is reproducible and is appropriate to uncover yet unknown protein/proteome function or properties using relevant model systems or publicly available data. Scope: -Fundamental studies in biology, including integrative "omics" studies, that provide mechanistic insights -Novel experimental and computational technologies -Proteogenomic data integration and analysis that enable greater understanding of physiology and disease processes -Pathway and network analyses of signaling that focus on the roles of post-translational modifications -Studies of proteome dynamics and quality controls, and their roles in disease -Studies of evolutionary processes effecting proteome dynamics, quality and regulation -Chemical proteomics, including mechanisms of drug action -Proteomics of the immune system and antigen presentation/recognition -Microbiome proteomics, host-microbe and host-pathogen interactions, and their roles in health and disease -Clinical and translational studies of human diseases -Metabolomics to understand functional connections between genes, proteins and phenotypes