Ultrabright ratiometric Raman-guided epilepsy surgery by intraoperatively visualizing proinflammatory microglia.

IF 11.7 1区医学 Q1 CELL BIOLOGY

Cell Reports Medicine Pub Date : 2025-06-17 Epub Date: 2025-05-30 DOI:10.1016/j.xcrm.2025.102155

Cong Wang, Zhi Li, Xiao Zhu, Wanbing Sun, Yue Ding, Wenjia Duan, Difei Wang, Yiqing Jiang, Ming Chen, Yuncan Chen, Jiayi Hu, Zheping Cai, Jing Zhao, Junfeng Wang, Zhen Fan, Faming Zheng, Xingyu Zhou, Fang Xie, Jianping Zhang, Yihui Guan, Kui Yan, Zuhai Lei, Qinyue Wang, Luting Wang, Xiao Xiao, Hairong Zheng, Liang Chen, Cong Li, Ying Mao

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引用次数: 0

Abstract

Resective surgery is an effective approach for long-term seizure control in drug-resistant focal epilepsy when the epileptic focus (EF) can be accurately delineated and removed. However, intraoperative mapping of EF with electrocorticography is laborious, time-consuming, and highly vulnerable to the effects of anesthesia. Here, we demonstrated that activated microglia can be reliable biomarkers for EF localization. Leveraging a newly developed ratiometric Raman nanosensor, ultraHOCls, we successfully visualize proinflammatory microglia in live epileptic mice, allowing for precise EF delineation without the interference of anesthesia. Compared to electrocorticography-guided surgery, ultraHOCl-guided surgery results in a substantial 61% reduction in total seizure burden in epileptic mouse models. Notably, ultraHOCls sprayed on freshly excised human brain tissues can effectively discriminate epileptic regions from non-epileptic tissues with high sensitivity (94.89%) and specificity (93.3%). This work provides an alternative strategy for delineating the EF intraoperatively, potentially revolutionizing surgery outcomes in epilepsy patients.

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术中观察促炎小胶质细胞的超亮比例拉曼引导癫痫手术。

切除手术是长期控制耐药局灶性癫痫发作的有效方法，可以准确地描绘和切除癫痫病灶。然而，术中使用皮质电图绘制EF是费力、耗时的，而且极易受到麻醉的影响。在这里，我们证明了激活的小胶质细胞可以作为EF定位的可靠生物标志物。利用新开发的比例拉曼纳米传感器ultraHOCls，我们成功地在活的癫痫小鼠中可视化促炎小胶质细胞，允许在没有麻醉干扰的情况下精确描绘EF。与皮质电图引导的手术相比，在癫痫小鼠模型中，超hocl引导的手术使总癫痫发作负担减少了61%。在新鲜切除的人脑组织上喷涂超hocls能有效区分癫痫区和非癫痫区，灵敏度（94.89%）和特异性（93.3%）均较高。这项工作为术中描述EF提供了一种替代策略，可能会彻底改变癫痫患者的手术结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Reports Medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

15.00

自引率

1.40%

发文量

231

审稿时长

40 days

期刊介绍： Cell Reports Medicine is an esteemed open-access journal by Cell Press that publishes groundbreaking research in translational and clinical biomedical sciences, influencing human health and medicine. Our journal ensures wide visibility and accessibility, reaching scientists and clinicians across various medical disciplines. We publish original research that spans from intriguing human biology concepts to all aspects of clinical work. We encourage submissions that introduce innovative ideas, forging new paths in clinical research and practice. We also welcome studies that provide vital information, enhancing our understanding of current standards of care in diagnosis, treatment, and prognosis. This encompasses translational studies, clinical trials (including long-term follow-ups), genomics, biomarker discovery, and technological advancements that contribute to diagnostics, treatment, and healthcare. Additionally, studies based on vertebrate model organisms are within the scope of the journal, as long as they directly relate to human health and disease.