RT-qPCR Quantification of Adipogenic Marker Genes (PPARg, C/EBPa, FABP4, and CD36).

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4607-6_6

Carina C Babbo, Reabetswe R Pitere, Rachel Giles, Melvin A Ambele, Michael S Pepper

引用次数: 0

Abstract

Adipogenesis is a complex multiphase process in which preadipocytes develop into adipocytes. This tightly regulated process is facilitated by a cascade of genes and transcription factors. In this chapter, we describe a protocol that can be used to quantify the expression of adipogenic markers using RT-qPCR.

查看原文本刊更多论文

脂肪生成标记基因（ppar, C/EBPa， FABP4和CD36）的RT-qPCR定量。

脂肪形成是一个复杂的多相过程，其中前脂肪细胞发育为脂肪细胞。这种严格调控的过程是由一系列基因和转录因子促进的。在本章中，我们描述了一种可以使用RT-qPCR来量化脂肪生成标记物表达的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.