Hamadou Dicko, Jéremie Vidal-Dupiol, Denis Saulnier, Eric Tambutté, Alexander Venn, Sylvie Tambutté, Virginie Chamard, Julien Duboisset
{"title":"3D Calcium carbonate polymorphs imaging with stimulated Raman scattering in biominerals.","authors":"Hamadou Dicko, Jéremie Vidal-Dupiol, Denis Saulnier, Eric Tambutté, Alexander Venn, Sylvie Tambutté, Virginie Chamard, Julien Duboisset","doi":"10.1039/d5fd00025d","DOIUrl":null,"url":null,"abstract":"<p><p>We present a highly sensitive coherent Raman microscopy approach, which allows for the tridimensional (3D) imaging of a series of carbonate polymorphs in marine organisms. CaCO<sub>3</sub> biomineralization occurs from the transformation of metastable amorphous precursors and other crystalline phases into a final crystalline phase. Understanding biomineralization pathways requires identifying this physico-chemical temporal sequence. Our approach exploits the different vibrational signatures of amorphous calcium carbonate, aragonite, calcite, Mg-calcite or hemi-hydrated calcium carbonate. This optical method enables the production of spatially and spectrally resolved images of the different compounds. When applied on the growing edge of post-mortem samples of both <i>Pinctada margaritifera</i> pearl oyster shell and <i>Stylophora pistillata</i> coral, it allows for inferring a temporal crystallisation sequence. We thus highlight the existence of intermediate crystalline phases, involving magnesian calcite or hemi-hydrated calcium carbonate, respectively.</p>","PeriodicalId":76,"journal":{"name":"Faraday Discussions","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Faraday Discussions","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d5fd00025d","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
We present a highly sensitive coherent Raman microscopy approach, which allows for the tridimensional (3D) imaging of a series of carbonate polymorphs in marine organisms. CaCO3 biomineralization occurs from the transformation of metastable amorphous precursors and other crystalline phases into a final crystalline phase. Understanding biomineralization pathways requires identifying this physico-chemical temporal sequence. Our approach exploits the different vibrational signatures of amorphous calcium carbonate, aragonite, calcite, Mg-calcite or hemi-hydrated calcium carbonate. This optical method enables the production of spatially and spectrally resolved images of the different compounds. When applied on the growing edge of post-mortem samples of both Pinctada margaritifera pearl oyster shell and Stylophora pistillata coral, it allows for inferring a temporal crystallisation sequence. We thus highlight the existence of intermediate crystalline phases, involving magnesian calcite or hemi-hydrated calcium carbonate, respectively.