Microwave-assisted Encapsulation of Cyclodextrin Metal-organic Frameworks (γ-CDMOF-1) Carrier for the Delivery of Quercetin and Curcumin against Lung Cancer with Improved Release Kinetics and Molecular Interaction Analysis

IF 2.7 3区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR

Inorganica Chimica Acta Pub Date : 2025-05-27 DOI:10.1016/j.ica.2025.122787

Saurelle Kenfack Tiofack , Patrice Kenfack Tsobnang , Muhammad Alif Mohamad Latif , Jean Ngoune , Mohd Basyaruddin Abdul Rahman

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引用次数: 0

Abstract

Polyphenol compounds like quercetin (QE) and curcumin (CCM) have demonstrated good potential in killing lung cancer cells, but their low physicochemical stability and poor aqueous solubility hinder their use. Herein, this study presents the synthesis and characterization of γ-Cyclodextrin Metal-Organic Framework (γ-CDMOF-1), a promising drug delivery system and an improving agent of the aqueous solubility of these bioactive molecules. Powder X-ray diffraction and Fourier-transform infrared spectroscopy confirmed the synthesis of γ-CDMOF-1 and the drug loading. High-resolution transmission electron microscopy and Field emission scanning electron microscopy showed the cubic shape of unloaded and loaded γ-CDMOF-1. Atomic force microscopy showed decreasing roughness, confirming the encapsulation of curcumin and quercetin inside the γ-CDMOF-1. Brunauer–Emmett–Teller (BET) results revealed that the surface area of γ-CDMOF-1, CCM@CDMOF-1, and QE@CDMOF-1 samples are 809, 541, and 359 m²/g, respectively. Encapsulation efficiency and loading capacity of CCM in γ-CDMOF-1 at 1000 μg/mL are (43.57 ± 1.71) %, (53.41 ± 1.9) mg/g, respectively, while those of QE are (65.35 ± 1.78) %, (91.36 ± 3.12) mg/g, respectively. Curcumin and quercetin are released from CCM@CDMOF-1 and QE@CDMOF-1 at least three times than curcumin and quercetin in the PBS and SLF media at pH 6.0 and 7.4. The solubility of CCM and QE loaded in γ-CDMOF-1 increases, nearly 2180-fold and 113-fold more than pure curcumin and quercetin, respectively. The molecular docking simulations showed that CCM@CDMOF-1 and QE@CDMOF-1 have the binding affinity of −9.2 kcal/mol and − 8.6 kcal/mol, respectively. Hydrogen bonds are mainly involved in the formation of both composite materials. The encapsulation of curcumin and quercetin into the γ-CDMOF-1 through a microwave-assisted method in the range of 100 to 1000 μg/mL improves the drug delivery and the water solubility of these bioactive molecules.

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微波辅助环糊精金属-有机框架（γ-CDMOF-1）载体包封抗肺癌槲皮素和姜黄素，改善释放动力学和分子相互作用分析

槲皮素（QE）和姜黄素（CCM）等多酚类化合物已显示出良好的杀伤肺癌细胞的潜力，但其物理化学稳定性和水溶性较差阻碍了它们的应用。本文介绍了γ-环糊精金属有机骨架（γ-CDMOF-1）的合成和表征，这是一种很有前途的药物传递系统，也是一种改善这些生物活性分子水溶性的剂。粉末x射线衍射和傅里叶变换红外光谱证实了γ-CDMOF-1的合成和载药。高分辨率透射电镜和场发射扫描电镜显示了加载和卸载的γ-CDMOF-1的立方形状。原子力显微镜显示粗糙度减小，证实姜黄素和槲皮素被包封在γ-CDMOF-1内。brunauer - emmet - teller （BET）结果显示，γ-CDMOF-1、CCM@CDMOF-1和QE@CDMOF-1样品的比表面积分别为809、541和359 m2/g。1000 μg/mL时，CCM在γ-CDMOF-1中的包封率和载药量分别为（43.57±1.71）%、（53.41±1.9）mg/g， QE的包封率和载药量分别为（65.35±1.78）%、（91.36±3.12）mg/g。在pH 6.0和7.4的PBS和SLF培养基中，CCM@CDMOF-1和QE@CDMOF-1中姜黄素和槲皮素的释放量至少是姜黄素和槲皮素的3倍。在γ-CDMOF-1中负载CCM和QE的溶解度分别比纯姜黄素和槲皮素高近2180倍和113倍。分子对接模拟表明，CCM@CDMOF-1和QE@CDMOF-1的结合亲和力分别为−9.2 kcal/mol和−8.6 kcal/mol。氢键主要参与了这两种复合材料的形成。通过微波辅助的方法将姜黄素和槲皮素包封在γ-CDMOF-1中，包封量在100 ~ 1000 μg/mL范围内，提高了这些生物活性分子的给药能力和水溶性。

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来源期刊

Inorganica Chimica Acta 化学-无机化学与核化学

CiteScore

6.00

自引率

3.60%

发文量

440

审稿时长

35 days

期刊介绍： Inorganica Chimica Acta is an established international forum for all aspects of advanced Inorganic Chemistry. Original papers of high scientific level and interest are published in the form of Articles and Reviews. Topics covered include: • chemistry of the main group elements and the d- and f-block metals, including the synthesis, characterization and reactivity of coordination, organometallic, biomimetic, supramolecular coordination compounds, including associated computational studies; • synthesis, physico-chemical properties, applications of molecule-based nano-scaled clusters and nanomaterials designed using the principles of coordination chemistry, as well as coordination polymers (CPs), metal-organic frameworks (MOFs), metal-organic polyhedra (MPOs); • reaction mechanisms and physico-chemical investigations computational studies of metalloenzymes and their models; • applications of inorganic compounds, metallodrugs and molecule-based materials. Papers composed primarily of structural reports will typically not be considered for publication.