Inhibitory effects of schisandrin A on contractions induced by spasmogenic candidates in porcine coronary arteries

Abstract

The inhibitory effects and underlying mechanisms of schisandrin A (SA) on contractions induced by spasmogenic candidates and related chemicals were investigated in porcine coronary arteries (PCAs). SA (10⁻⁵–10⁻⁴ M) inhibited contractions induced by acetylcholine, histamine, serotonin, U46619 (thromboxane A₂ mimetic), prostaglandin F_2α, and endothelin-1 in a concentration-dependent manner. The inhibition of acetylcholine-induced contractions by SA was stronger than that by diltiazem, although both SA and diltiazem ultimately achieved similar levels of inhibition against other contractions. SA also inhibited high-KCl-induced contractions in PCAs and suppressed high-KCl-induced increases in intracellular Ca²⁺ concentrations in A7r5 cells. However, SA (10⁻⁴ M) did not inhibit SKF-96365-sensitive phenylephrine-induced contractions, despite potently inhibiting high-KCl-induced contraction in the guinea pig thoracic aorta. SA did not strongly inhibit NaF-induced contractions in Ca²⁺-free solution containing 0.2 mM EGTA. Furthermore, SA inhibited muscarinic receptor binding in mouse cerebral cortex and inhibited carbachol-induced increases in intracellular Ca²⁺ concentrations in 293T cells expressing muscarinic M₃ receptors. These findings indicate that SA inhibits coronary artery contractions induced by spasmogens primarily through the inhibition of L-type Ca²⁺ channels (LCCs) and exerts an anticholinergic and LCC inhibitory effect on acetylcholine-induced contractions.