Characterization of paralogous uncx transcription factor encoding genes in zebrafish.

Q3 Biochemistry, Genetics and Molecular Biology

Gene: X Pub Date : 2019-06-01 DOI:10.1016/j.gene.2019.100011

Valeria Nittoli, Antonio Emidio Fortunato, Giulia Fasano, Ugo Coppola, Alessandra Gentile, Sylvie Maiella, Fernanda Langellotto, Immacolata Porreca, Raffaella De Paolo, Rita Marino, Marcella Fiengo, Aldo Donizetti, Francesco Aniello, Takashi Kondo, Filomena Ristoratore, Lorella M T Canzoniero, Denis Duboule, Stephen W Wilson, Paolo Sordino

{"title":"Characterization of paralogous uncx transcription factor encoding genes in zebrafish.","authors":"Valeria Nittoli, Antonio Emidio Fortunato, Giulia Fasano, Ugo Coppola, Alessandra Gentile, Sylvie Maiella, Fernanda Langellotto, Immacolata Porreca, Raffaella De Paolo, Rita Marino, Marcella Fiengo, Aldo Donizetti, Francesco Aniello, Takashi Kondo, Filomena Ristoratore, Lorella M T Canzoniero, Denis Duboule, Stephen W Wilson, Paolo Sordino","doi":"10.1016/j.gene.2019.100011","DOIUrl":null,"url":null,"abstract":"The paired-type homeodomain transcription factor Uncx is involved in multiple processes of embryogenesis in vertebrates. Reasoning that zebrafish genes uncx4.1 and uncx are orthologs of mouse Uncx, we studied their genomic environment and developmental expression. Evolutionary analyses indicate the zebrafish uncx genes as being paralogs deriving from teleost-specific whole-genome duplication. Whole-mount in situ mRNA hybridization of uncx transcripts in zebrafish embryos reveals novel expression domains, confirms those previously known, and suggests sub-functionalization of paralogs. Using genetic mutants and pharmacological inhibitors, we investigate the role of signaling pathways on the expression of zebrafish uncx genes in developing somites. In identifying putative functional role(s) of zebrafish uncx genes, we hypothesized that they encode transcription factors that coordinate growth and innervation of somitic muscles.","PeriodicalId":37140,"journal":{"name":"Gene: X","volume":"2 ","pages":"100011"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6543554/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene: X","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.gene.2019.100011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

Abstract

The paired-type homeodomain transcription factor Uncx is involved in multiple processes of embryogenesis in vertebrates. Reasoning that zebrafish genes uncx4.1 and uncx are orthologs of mouse Uncx, we studied their genomic environment and developmental expression. Evolutionary analyses indicate the zebrafish uncx genes as being paralogs deriving from teleost-specific whole-genome duplication. Whole-mount in situ mRNA hybridization of uncx transcripts in zebrafish embryos reveals novel expression domains, confirms those previously known, and suggests sub-functionalization of paralogs. Using genetic mutants and pharmacological inhibitors, we investigate the role of signaling pathways on the expression of zebrafish uncx genes in developing somites. In identifying putative functional role(s) of zebrafish uncx genes, we hypothesized that they encode transcription factors that coordinate growth and innervation of somitic muscles.

查看原文本刊更多论文

斑马鱼同源unx转录因子编码基因的研究。

配对型同源结构域转录因子Uncx参与脊椎动物胚胎发生的多个过程。鉴于斑马鱼的uncx4.1和uncx基因与小鼠的uncx基因同源，我们研究了它们的基因组环境和发育表达。进化分析表明，斑马鱼unx基因是源自硬骨鱼特异性全基因组复制的类似物。斑马鱼胚胎中unx转录本的全安装原位mRNA杂交揭示了新的表达域，证实了先前已知的表达域，并提示了类似物的亚功能化。利用基因突变体和药物抑制剂，我们研究了信号通路在斑马鱼发育体中uncx基因表达中的作用。在确定斑马鱼unx基因的假定功能作用时，我们假设它们编码协调体肌生长和神经支配的转录因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gene: X Biochemistry, Genetics and Molecular Biology-Genetics

自引率

0.00%

发文量