Vanari Gutika supplementation enhances testicular function by modulating spermatogenesis, oxidative balance, and blood-testis barrier integrity.

Abstract

Objectives: Vanari Gutika (VG) is an ayurvedic formulation that has been traditionally utilized for the treatment of various male sexual problems. The primary components of VG include Mucuna pruriens, honey, and clarified butter, which are recognized for their aphrodisiac properties. However, currently, there is no scientific evidence supporting the use of this formulation as a drug for enhancing male fertility or elucidating its mechanism for improving testicular physiology. This study aimed to examine the effect of VG on spermatogenesis.

Methods: Adult male mice received oral administration of VG at doses of 75, 150, and 300 mg/kg body weight daily for 35 days (one spermatogenic cycle). Parameters such as histomorphology of testes, daily sperm production, sperm parameters, activity of antioxidant enzymes (SOD and catalase), and immunoblotting of BTB marker proteins (N-cadherin, connexin 43, β-catenin) were assessed.

Results: VG treatment markedly improves relative testis weight, DSP, and sperm count compared to control. The administration of VG resulted in significant improvements in testis histomorphology, germ cell proliferation, and anti-oxidant enzymes (increased catalase and SOD activities) when compared to control mice. Additionally, there was also a dose-dependent increase in the expression of BTB junctional proteins. VG treatment (150 and 300 mg/kg BW) showed a significant beneficial impact on spermatogenesis in adult male mice.

Conclusions: Thus, our findings strongly support the potent therapeutic potential of Vanari Gutika as an ayurvedic aphrodisiac in enhancing testicular function.