Stefany Cristine Rodrigues da Silva, Francisco Meirelles Bastos de Oliveira
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引用次数: 0
Abstract
In Saccharomyces cerevisiae, the cyclin-dependent kinase Bur1 is primarily known for its role in promoting transcription elongation, thereby regulating gene expression. In this study, we investigated the genetic interactions between a hypomorphic BUR1 allele (bur1-107) and null mutants of the checkpoint kinases Mec1 and Rad53. Remarkably, bur1-107 alleviated the sensitivity of mec1 and rad53 mutants to hydroxyurea (HU), suggesting that Bur1 activity becomes detrimental when checkpoint signaling is impaired. Furthermore, the bur1-107 mutation delayed the G1-to-S phase transition, implicating Bur1 as a key player in cell cycle progression. In HU-treated mec1 mutants, bur1-107 reduced γ-H2A accumulation, promoted S-phase resumption, and suppressed the formation of sub-G1 populations. Together, these findings suggest that Bur1-driven G1-to-S phase progression exacerbates DNA damage and cell death in checkpoint-deficient cells exposed to HU. This study highlights a novel role for Bur1 in modulating the cellular response to replication stress in checkpoint-compromised cells.
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GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work.
While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal.
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GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.
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