Copy Number Determination of Sperm-Borne Small RNAs Implied in the Intergenerational Inheritance of Metabolic Syndromes.

IF 4.2 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
RNA Pub Date : 2025-05-28 DOI:10.1261/rna.080480.125
Lisa Koenig, Victoria Guggenberger, Kristeli Eleftheriou, Zsuzsanna Pinter, Alessandro Marotto, Christoph R Kreutz, Mark Wossidlo, Virginie Marchand, Yuri Motorin, Matthias R Schaefer
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引用次数: 0

Abstract

Mammalian spermatocytes harbour small RNAs that are mostly degradation products of abundant non-coding RNAs, including ribosomal RNA-derived small RNAs (rsRNAs) and tRNA-derived RNAs (tDRs). Notably, tDRs have been implicated in inheriting paternally acquired traits in rodents. Direct experimental proof for this notion comes from manipulating fertilized murine oocytes through microinjection of small RNA preparations, resulting in metabolic changes measurable in the offspring. How these paternally transmitted small RNAs could function mechanistically in the developing zygote remains to be understood. Since nothing is known about how many small RNAs are required for functional impact, we aimed to determine the copy numbers of specific small RNAs contained in a single spermatocyte. Using hybridization-based methods that avoid amplification-induced biases, we estimated average copy numbers for specific tDRs and rsRNAs per murine spermatocyte. While the measured numbers allow an approximation of how many rRNA- and tRNA-derived RNAs enter a murine oocyte during fertilization, the magnitude of these numbers underscores the need for remaining cautious when interpreting the effects of non-physiological copy numbers of small RNAs that were used to manipulate a biological system.

代谢综合征代际遗传中精子携带小rna的拷贝数测定。
哺乳动物精细胞中含有小rna,这些小rna主要是大量非编码rna的降解产物,包括核糖体rna衍生的小rna (rsRNAs)和trna衍生的rna (tDRs)。值得注意的是,在啮齿类动物中,tdr与继承父系获得的特征有关。这一观点的直接实验证据来自于通过微注射小RNA制剂操纵受精的小鼠卵母细胞,导致后代可测量的代谢变化。这些父系遗传的小rna如何在发育中的受精卵中发挥机制作用仍有待了解。由于对功能影响需要多少小rna尚不清楚,我们的目标是确定单个精母细胞中包含的特定小rna的拷贝数。使用基于杂交的方法避免扩增引起的偏差,我们估计了每个小鼠精母细胞中特定tdr和rsRNAs的平均拷贝数。虽然测量的数字可以近似地了解在受精过程中有多少rRNA和trna衍生的rna进入小鼠卵母细胞,但这些数字的大小强调了在解释用于操纵生物系统的小rna的非生理拷贝数的影响时保持谨慎的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
RNA
RNA 生物-生化与分子生物学
CiteScore
8.30
自引率
2.20%
发文量
101
审稿时长
2.6 months
期刊介绍: RNA is a monthly journal which provides rapid publication of significant original research in all areas of RNA structure and function in eukaryotic, prokaryotic, and viral systems. It covers a broad range of subjects in RNA research, including: structural analysis by biochemical or biophysical means; mRNA structure, function and biogenesis; alternative processing: cis-acting elements and trans-acting factors; ribosome structure and function; translational control; RNA catalysis; tRNA structure, function, biogenesis and identity; RNA editing; rRNA structure, function and biogenesis; RNA transport and localization; regulatory RNAs; large and small RNP structure, function and biogenesis; viral RNA metabolism; RNA stability and turnover; in vitro evolution; and RNA chemistry.
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