Synthesis, characterization and evaluation of linoleic acid-loaded nano-carriers: a novel drug delivery system for efficient treatment of hepatocellular carcinoma.

IF 2.2 4区 医学 Q3 ONCOLOGY
Anti-Cancer Drugs Pub Date : 2025-09-01 Epub Date: 2025-05-30 DOI:10.1097/CAD.0000000000001734
Mahsa Ghasemzad, Haleh Bakhshandeh, Roghayeh Naserkhaki, Ibrahim Ghoytasi, Bahare Shokoohian, Alireza Fotouhi, Zohreh Miri-Lavasani, Elham Rismani, Nikoo Hossein-Khannazer, Abbas Piryaei, Massoud Vosough
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引用次数: 0

Abstract

Hepatocellular carcinoma is the main primary liver cancer. Due to the high recurrence rate and potential resistance to treatments, there is an urgent need to find more effective and targeted therapies. Combinational therapies and using advanced drug delivery methods are promising approaches. Niosomes are one of the popular types of nanoparticles in modern drug delivery systems and have recently attracted more attention. They are biodegradable, nonimmunogenic, and more stable carriers than liposomes. They can release anticancer drugs in an efficient and controlled manner. The aim of this study was to synthesize and characterize the physicochemical properties of linoleic acid (LA)-loaded niosomes and evaluate their anticancer effects on a hepatoma cell. We synthesized LA-loaded niosomes using a thin-film hydration method and characterized their size, polydispersity index (PDI), and zeta potential. The morphology of niosomes was evaluated by scanning electron microscopy. Finally, the viability, migration capacity, and colonization potential of Hep-3B hepatoma cells treated with 150 μM LA-loaded niosomes were investigated and compared to the control groups. The niosomes had a mean size of 266.9 nm, PDI of 0.271, and zeta potential of -26.1 mV. The LA-loaded niosomes released LA sustainably at 37 °C for 72 h. MTS assay indicated that the LA-loaded niosomes were more toxic than free LA. Hep-3B hepatoma cells treated with LA-loaded niosomes showed a remarkable decline in the migration ability and colony-formation capacity. Therefore, the use of LA-loaded niosomes in combination with conventional protocols may be a promising approach to target cancer cells.

载亚油酸纳米载体的合成、表征和评价:一种有效治疗肝细胞癌的新型药物传递系统。
肝细胞癌是主要的原发性肝癌。由于其高复发率和潜在的耐药性,迫切需要寻找更有效和更有针对性的治疗方法。联合治疗和使用先进的给药方法是很有前途的方法。乳小体是现代给药系统中常用的纳米颗粒之一,近年来受到越来越多的关注。它们是可生物降解的,非免疫原性的,比脂质体更稳定的载体。它们可以有效和可控地释放抗癌药物。本研究的目的是合成和表征亚油酸(LA)负载ni质体的理化性质,并评价其对肝癌细胞的抗癌作用。我们采用薄膜水合法合成了负载la的niosomes,并对其尺寸、多分散性指数(PDI)和zeta电位进行了表征。用扫描电镜观察纳米体的形态。最后,研究了负载150 μM la的niosome处理Hep-3B肝癌细胞的活力、迁移能力和定植潜力,并与对照组进行了比较。所得小体平均尺寸为266.9 nm, PDI为0.271,zeta电位为-26.1 mV。负载LA的niosome在37°C下持续释放LA 72 h。MTS实验表明负载LA的niosome比游离LA的毒性更大。负载la的niosomes处理的Hep-3B肝癌细胞的迁移能力和集落形成能力显著下降。因此,将负载la的尼奥体与常规方案结合使用可能是一种很有前途的靶向癌细胞的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Anti-Cancer Drugs
Anti-Cancer Drugs 医学-药学
CiteScore
3.80
自引率
0.00%
发文量
244
审稿时长
3 months
期刊介绍: Anti-Cancer Drugs reports both clinical and experimental results related to anti-cancer drugs, and welcomes contributions on anti-cancer drug design, drug delivery, pharmacology, hormonal and biological modalities and chemotherapy evaluation. An internationally refereed journal devoted to the fast publication of innovative investigations on therapeutic agents against cancer, Anti-Cancer Drugs aims to stimulate and report research on both toxic and non-toxic anti-cancer agents. Consequently, the scope on the journal will cover both conventional cytotoxic chemotherapy and hormonal or biological response modalities such as interleukins and immunotherapy. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool.
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