Molecular detection of benzimidazole resistance in Haemonchus contortus: A comparative analysis of field and abattoir larval isolates from sheep and goats

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Small Ruminant Research Pub Date : 2025-05-28 DOI:10.1016/j.smallrumres.2025.107555

Mohamed A. Helal , Prashant D. Pawar , Lachhman Das Singla

{"title":"Molecular detection of benzimidazole resistance in Haemonchus contortus: A comparative analysis of field and abattoir larval isolates from sheep and goats","authors":"Mohamed A. Helal , Prashant D. Pawar , Lachhman Das Singla","doi":"10.1016/j.smallrumres.2025.107555","DOIUrl":null,"url":null,"abstract":"<div><div>Benzimidazole (BZ) resistance in <em>Haemonchus contortus</em> presents a significant threat to small ruminant production, particularly in developing countries. This study employed molecular techniques to detect BZ resistance in <em>H. contortus</em> larvae isolated from sheep and goats in both field and abattoir settings. Using allele-specific PCR (AS-PCR), we analysed polymorphisms in codon 200 of the β-tubulin isotype 1 gene, complemented by restriction fragment length polymorphism (RFLP) analysis for species verification. In goat larval isolates (n = 156), genotypic analysis revealed 58 % homozygous resistant (RR), 12 % homozygous susceptible (SS), and 30 % heterozygous (RS) larvae. Sheep larval isolates (n = 218) showed a different distribution: 29 % RR, 20 % SS, and 51 % RS.The overall frequency of the resistance allele (R)was notably higher in goat samples (73 %) compared to sheep samples (54 %). These findings indicate widespread BZ resistance in both host species, with a particularly concerning prevalence in goat populations. This study demonstrates the utility of molecular techniques for early resistance detection and highlights the need for targeted anthelmintic management strategies in small ruminant production systems.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"250 ","pages":"Article 107555"},"PeriodicalIF":1.6000,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small Ruminant Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0921448825001282","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Benzimidazole (BZ) resistance in Haemonchus contortus presents a significant threat to small ruminant production, particularly in developing countries. This study employed molecular techniques to detect BZ resistance in H. contortus larvae isolated from sheep and goats in both field and abattoir settings. Using allele-specific PCR (AS-PCR), we analysed polymorphisms in codon 200 of the β-tubulin isotype 1 gene, complemented by restriction fragment length polymorphism (RFLP) analysis for species verification. In goat larval isolates (n = 156), genotypic analysis revealed 58 % homozygous resistant (RR), 12 % homozygous susceptible (SS), and 30 % heterozygous (RS) larvae. Sheep larval isolates (n = 218) showed a different distribution: 29 % RR, 20 % SS, and 51 % RS.The overall frequency of the resistance allele (R)was notably higher in goat samples (73 %) compared to sheep samples (54 %). These findings indicate widespread BZ resistance in both host species, with a particularly concerning prevalence in goat populations. This study demonstrates the utility of molecular techniques for early resistance detection and highlights the need for targeted anthelmintic management strategies in small ruminant production systems.

查看原文本刊更多论文

扭曲血蜱对苯并咪唑耐药性的分子检测：绵羊和山羊田间和屠宰场幼虫分离物的比较分析

弯曲血蜱对苯并咪唑（BZ）的耐药性对小反刍动物生产构成重大威胁，特别是在发展中国家。本研究采用分子技术检测田间和屠宰场分离的绵羊和山羊弯纹夜蛾幼虫对BZ的抗性。利用等位基因特异性PCR （AS-PCR）分析了β-微管蛋白同型1基因密码子200的多态性，并结合限制性片段长度多态性（RFLP）分析进行物种验证。在山羊幼虫分离株（n = 156）中，基因型分析显示58 %纯合子抗性（RR）， 12 %纯合子敏感（SS）和30 %杂合子（RS）幼虫。绵羊幼虫分离株（n = 218）的抗性等位基因总频率分别为29 % RR、20 % SS和51 % RR，其中山羊的抗性等位基因总频率（73 %）明显高于绵羊（54 %）。这些发现表明，BZ耐药性在两种宿主物种中广泛存在，在山羊种群中的流行尤其令人担忧。这项研究证明了分子技术在早期耐药性检测中的实用性，并强调了在小型反刍动物生产系统中有针对性的驱虫管理策略的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Small Ruminant Research 农林科学-奶制品与动物科学

CiteScore

3.10

自引率

11.10%

发文量

210

审稿时长

12.5 weeks

期刊介绍： Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.