Gut Microbiota Dysbiosis in Japanese Female Patients with Nontuberculous Mycobacteria-Associated Lung Disease: An Observational Study.

IF 3.9 3区 工程技术 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Kanako Kono, Yutaka Kozu, Shun Yokota, Kouta Hatayama, Kenji Mizumura, Shuichiro Maruoka, Hiroaki Masuyama, Yasuhiro Gon
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Abstract

Background/Objectives: Nontuberculous mycobacterial pulmonary disease (NTM-PD) is treated using a combination of multiple antimicrobial agents and prolonged therapy; however, recurrence and reinfection rates remain high. Susceptibility to NTM-PD is not fully understood. We aimed to investigate the association between NTM-PD and gut microbiota and determine the impact of antimicrobial therapy on the composition of the gut microbiota. Methods: We analyzed the gut microbiota of 20 Japanese females with NTM-PD (mean age: 67.9 years; range: 50-80 years) at different treatment stages-before, during, and at recurrence-alongside 20 healthy individuals, using 16S rRNA gene amplicon sequencing. Results: Subgroup A (pre-treatment) showed a small difference in β-diversity when compared with the healthy control (HC) group, while no significant differences in α-diversity were observed. Subgroup B (during treatment) exhibited a larger difference in β-diversity compared with the HC group, along with a decrease in α-diversity. The α-diversity of the gut microbiota in Subgroup C (at recurrence) was lower than that in Subgroup A but higher than that in Subgroup B. In Subgroups A and C, the bacterial taxa Sutterella, Adlercreutzia, Odoribacter, and Prevotella had decreased relative abundance, while Erysipelatoclostridium, Massilimicrobiota, Flavonifractor, Eggerthella, and Fusobacterium had increased relative abundance compared to those in the HC group. Conclusions: The loss of normal resident gut bacteria may hinder reacquisition. Treatment may be associated with the persistence of a dysbiotic gut microbiota, fostering susceptibility to NTM-PD. Gut microbiota dysbiosis may heighten susceptibility to NTM-PD, complicate treatment outcomes, and increase the risk of microbiological recurrence following therapy.

日本女性非结核分枝杆菌相关肺病患者肠道菌群失调:一项观察性研究
背景/目的:非结核性分枝杆菌肺病(NTM-PD)的治疗采用多种抗菌药物联合长期治疗;然而,复发率和再感染率仍然很高。对NTM-PD的易感性尚未完全了解。我们旨在研究NTM-PD与肠道微生物群之间的关系,并确定抗菌治疗对肠道微生物群组成的影响。方法:我们分析了20名日本NTM-PD女性患者的肠道微生物群(平均年龄:67.9岁;范围:50-80岁),在不同的治疗阶段——复发前、复发中和复发时——与20名健康个体一起,使用16S rRNA基因扩增子测序。结果:A亚组(预处理)β-多样性与健康对照(HC)组比较差异较小,α-多样性差异无统计学意义。B亚组(治疗期间)β-多样性较HC组差异较大,α-多样性降低。C亚组(复发时)肠道菌群α-多样性低于A亚组,高于b亚组。A亚组和C亚组中,Sutterella、Adlercreutzia、Odoribacter和Prevotella菌群的相对丰度降低,而Erysipelatoclostridium、Massilimicrobiota、黄酮因子、Eggerthella和Fusobacterium的相对丰度高于HC组。结论:正常常驻肠道细菌的丧失可能会阻碍再获得。治疗可能与持续的肠道菌群失调有关,促进对NTM-PD的易感性。肠道菌群失调可能增加NTM-PD的易感性,使治疗结果复杂化,并增加治疗后微生物复发的风险。
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来源期刊
Biomedicines
Biomedicines Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
CiteScore
5.20
自引率
8.50%
发文量
2823
审稿时长
8 weeks
期刊介绍: Biomedicines (ISSN 2227-9059; CODEN: BIOMID) is an international, scientific, open access journal on biomedicines published quarterly online by MDPI.
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