Determining the FY*BES Allele in Iranian Sickle Cell Disease Patients to Enhance Matching Blood Transfusion.

Mina Samadi Ivriq, Arezoo Oodi, Saeed Mohammadi, Bijan Keikhaei-Dehdazi, Moharram Ahmadnezhad, Sahar Jolharnejad
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Abstract

Background: Duffy antibodies play a significant role in hemolytic transfusion reactions and hemolytic disease of the fetus and newborn, Duffy(FY) blood group genotyping an essential part of transfusion medicine. The purpose of this study was to assess the importance of Duffy (FY) DNA typing in conducting transfusion compatibility testing and improving Red Blood Cell matching during transfusion. Materials and Methods: In this study, 135 blood samples from SCD patients from the Southwest of Iran were included. All samples were tested with Anti-Fya and Anti-Fyb using the hemagglutination technique, and 64 samples with the fy(a+b-) and fy(a-b-) phenotypes were genotyped using DNA sequencing methods. Results: The prevalence of alloimmunization in this population was 13.04%. fy(a-b+) was the most common phenotype (37/135, 27.4%), followed by fy(a+b-) (35/135, 26%), fy(a+b+) (34/135, 25.2%); and fy(a-b-) (29/135, 21.4%). Among the 64 fy(a+b-) and fy(a-b-) samples, 40 (62.5%) patients had FY*BES allele. 21 out of 40 samples were FY*BES/FY*BES, 17 were FY*A/FY*BES, and 2 were FY*B/FY*BES. Conclusion: The prevalence of GATA-1 mutation (FY*BES allele), in fy(a-b-) and fy(a+b-) patients was reported 62.5%. Therefore, it is possible to use the genotypic information as a database to facilitate the process of searching and supplying better-matched blood transfusion.

伊朗镰状细胞病患者FY*BES等位基因检测提高输血配型
背景:达菲抗体在胎儿和新生儿的溶血性输血反应和溶血性疾病中起着重要作用,达菲血型基因分型是输血医学的重要组成部分。本研究的目的是评估达菲(FY) DNA分型在进行输血相容性测试和改善输血过程中的红细胞匹配中的重要性。材料和方法:本研究纳入了伊朗西南部SCD患者的135份血液样本。采用血凝技术对所有样品进行Anti-Fya和Anti-Fyb检测,并采用DNA测序方法对64份具有fy(a+b-)和fy(a-b-)表型的样品进行基因分型。结果:该人群异体免疫检出率为13.04%。Fy (a-b+)是最常见的表型(37/135,27.4%),其次是Fy (a+b-) (35/135, 26%), Fy (a+b+) (34/135, 25.2%);还有fy(a-b-) (29/ 135,21.4%)64例fy(a+b-)和fy(a-b-)样本中,40例(62.5%)患者携带fy *BES等位基因。40例样本中,FY*BES/FY*BES 21例,FY*A/FY*BES 17例,FY*B/FY*BES 2例。结论:GATA-1突变(FY*BES等位基因)在FY (a-b-)和FY (a+b-)患者中的患病率为62.5%。因此,有可能使用基因型信息作为数据库,以促进搜索和提供更好匹配的输血过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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