Mass-spectrometry based metabolomics: an overview of workflows, strategies, data analysis and applications.

IF 1.6 3区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Proteome Science Pub Date : 2025-05-26 DOI:10.1186/s12953-025-00241-8

Kosar Hajnajafi, Mohammad Askandar Iqbal

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引用次数: 0

Abstract

Background: Metabolomics, a burgeoning field within systems biology, focuses on the comprehensive study of small molecules present in biological systems. Mass spectrometry (MS) has emerged as a powerful tool for metabolomic analysis due to its high sensitivity, resolution, and ability to characterize a wide range of metabolites thus offering deep insights into the metabolic profiles of living systems.

Aim of review: This review provides an overview of the methodologies, workflows, strategies, data analysis techniques, and applications associated with mass spectrometry-based metabolomics.

Key scientific concepts of review: We discuss workflows, key strategies, experimental procedures, data analysis techniques, and diverse applications of metabolomics in various research domains. Nuances of sample preparation, metabolite extraction, separation using chromatographic techniques, mass spectrometry analysis, and data processing are elaborated. Moreover, standards, quality controls, metabolite annotation, software for statistical and pathway analysis are also covered. In conclusion, this review aims to facilitate the understanding and adoption of mass spectrometry-based metabolomics by newcomers and researchers alike by providing a foundational understanding and insights into the current state and future directions of this dynamic field.

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质谱代谢组学：工作流程、策略、数据分析和应用概述。

背景：代谢组学是系统生物学中的一个新兴领域，主要研究生物系统中存在的小分子。质谱（MS）已成为代谢组学分析的强大工具，因为它具有高灵敏度，分辨率和表征广泛代谢物的能力，从而提供了对生命系统代谢谱的深入了解。综述目的：本文综述了以质谱为基础的代谢组学的方法、工作流程、策略、数据分析技术和应用。综述的关键科学概念：我们讨论了工作流程，关键策略，实验程序，数据分析技术，以及代谢组学在不同研究领域的不同应用。样品制备，代谢物提取，分离使用色谱技术，质谱分析和数据处理的细微差别进行了阐述。此外，还包括标准，质量控制，代谢物注释，统计和途径分析软件。总之，本文旨在通过对这一动态领域的现状和未来发展方向提供基本的理解和见解，促进新人和研究人员对基于质谱的代谢组学的理解和采用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Proteome Science 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： Proteome Science is an open access journal publishing research in the area of systems studies. Proteome Science considers manuscripts based on all aspects of functional and structural proteomics, genomics, metabolomics, systems analysis and metabiome analysis. It encourages the submissions of studies that use large-scale or systems analysis of biomolecules in a cellular, organismal and/or environmental context. Studies that describe novel biological or clinical insights as well as methods-focused studies that describe novel methods for the large-scale study of any and all biomolecules in cells and tissues, such as mass spectrometry, protein and nucleic acid microarrays, genomics, next-generation sequencing and computational algorithms and methods are all within the scope of Proteome Science, as are electron topography, structural methods, proteogenomics, chemical proteomics, stem cell proteomics, organelle proteomics, plant and microbial proteomics. In spite of its name, Proteome Science considers all aspects of large-scale and systems studies because ultimately any mechanism that results in genomic and metabolomic changes will affect or be affected by the proteome. To reflect this intrinsic relationship of biological systems, Proteome Science will consider all such articles.