Aaptamine Alters Vimentin Expression and Migration Capability of Triple-Negative Breast Cancer Cells.

Q2 Medicine

Journal of Experimental Pharmacology Pub Date : 2025-05-22 eCollection Date: 2025-01-01 DOI:10.2147/JEP.S512099

Astrid Feinisa Khairani, Widad Aghnia Shalannandia, Muhammad Hasan Bashari, Nur Atik

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Abstract

Purpose: This study aimed to explore the effect of Aaptamine, an alkaloid derived from marine sponges, on the vimentin expression in both mRNA and protein levels and the migration capacity of breast cancer cells.

Methods: The triple-negative breast cancer cell line MDA-MB-231 was used for in vitro experiments. Low-cytotoxicity concentrations of Aaptamine (12.5 to 50 μM) were given to MDA-MB-231 cells. The vimentin mRNA and protein expression were evaluated using RT-qPCR and immunofluorescence, respectively, 72 h after Aaptamine treatment. The migration scratch assay was conducted for 48 hours.

Results: Aaptamine treatment in three different doses did not affect the expression of vimentin at the mRNA level while significantly lowering vimentin protein expression at the concentration of 12.5 µM. In addition, Aaptamine significantly inhibited breast cancer cell migration in a dose-dependent manner.

Conclusion: Aaptamine inhibits vimentin protein expression and demonstrates anti-migration activity in the sub-cytotoxic dose.

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apapamine改变三阴性乳腺癌细胞Vimentin表达和迁移能力。

目的：探讨海绵生物碱Aaptamine对乳腺癌细胞vimentin mRNA和蛋白表达及迁移能力的影响。方法：采用三阴性乳腺癌细胞株MDA-MB-231进行体外实验。MDA-MB-231细胞给予低浓度（12.5 ~ 50 μM）的Aaptamine。应用RT-qPCR和免疫荧光法分别检测Aaptamine处理72h后vimentin mRNA和蛋白的表达。迁移划痕实验进行48小时。结果：三种不同剂量的Aaptamine处理在mRNA水平上不影响vimentin的表达，但在12.5µM浓度下显著降低vimentin蛋白的表达。此外，Aaptamine显著抑制乳腺癌细胞迁移呈剂量依赖性。结论：亚细胞毒剂量下，Aaptamine抑制vimentin蛋白表达，具有抗迁移活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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