Luciano A Palomino-Kobayashi, Rocío Egoávil-Espejo, Gina Salvador-Luján, Pamela Yáñez, Ronnie G Gavilán, Maria J Pons, Joaquim Ruiz
{"title":"Detection of rare β-lactamase <i>bla</i> <sub>SCO-1</sub> from a <i>Klebsiella pneumoniae</i> high-risk clone in Peru.","authors":"Luciano A Palomino-Kobayashi, Rocío Egoávil-Espejo, Gina Salvador-Luján, Pamela Yáñez, Ronnie G Gavilán, Maria J Pons, Joaquim Ruiz","doi":"10.1093/jacamr/dlaf089","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The <i>bla</i> <sub>SCO-1</sub> gene, which codes for a carbenicillinase, is an uncommon β-lactamase. To the best of our knowledge, we report the first instance of a <i>Klebsiella pneumoniae</i> clinical high-risk isolate carrying <i>bla</i> <sub>SCO-1</sub> in Peru.</p><p><strong>Objectives: </strong>To characterize a <i>K. pneumoniae</i> clinical isolate carrying <i>bla</i> <sub>SCO-1</sub> in Peru, isolated from a tertiary care hospital in Lima, Peru.</p><p><strong>Methods: </strong>Susceptibility to amoxicillin/clavulanic acid, amoxicillin/sulbactam, piperacillin/tazobactam, aztreonam, ceftriaxone, cefotaxime, ceftazidime, ceftazidime/avibactam, cefepime, imipenem, meropenem, ciprofloxacin, trimethoprim/sulfamethoxazole, gentamicin, amikacin and tigecycline was determined using the Kirby-Bauer disc diffusion method. Detection of <i>bla</i> <sub>SCO-1</sub> was established by Illumina WGS and bioinformatic analyses. All the publicly available genomes from <i>K. pneumoniae</i> carrying <i>bla</i> <sub>SCO-1</sub> from the American continent were downloaded from the NCBI Isolates browser for assessment of phylogenetic comparisons.</p><p><strong>Results: </strong>The isolate showed resistance to all the tested antibiotics except for ceftazidime/avibactam, carbapenems and tigecycline. Illumina WGS showed the presence of <i>bla</i> <sub>SCO-1</sub> and <i>bla</i> <sub>OXA-1</sub>, <i>bla</i> <sub>SHV-28</sub>, <i>bla</i> <sub>TEM-1</sub>, <i>bla</i> <sub>CTX-M-15</sub>, as well as genes associated with resistance to quinolones, fosfomycin, aminoglycosides, sulphonamides, trimethoprim and phenicols. Moreover, the isolate was ST307, a high-risk clone of <i>K. pneumoniae</i> usually associated with <i>bla</i> <sub>CTX-M-15</sub> and/or carbapenemases. Notwithstanding, the latter were not found on this isolate. Phylogenetic relationships were established by comparisons with 19 <i>K. pneumoniae</i> genomes carrying <i>bla</i> <sub>SCO-1</sub> from other countries in the Americas, revealing at least three different clades.</p><p><strong>Conclusions: </strong>This study highlights the importance of genomic surveillance of uncommon antimicrobial resistance genes such as <i>bla</i> <sub>SCO-1</sub>, which might contribute to further antimicrobial resistance levels in this country.</p>","PeriodicalId":14594,"journal":{"name":"JAC-Antimicrobial Resistance","volume":"7 3","pages":"dlaf089"},"PeriodicalIF":3.3000,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103947/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"JAC-Antimicrobial Resistance","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/jacamr/dlaf089","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The blaSCO-1 gene, which codes for a carbenicillinase, is an uncommon β-lactamase. To the best of our knowledge, we report the first instance of a Klebsiella pneumoniae clinical high-risk isolate carrying blaSCO-1 in Peru.
Objectives: To characterize a K. pneumoniae clinical isolate carrying blaSCO-1 in Peru, isolated from a tertiary care hospital in Lima, Peru.
Methods: Susceptibility to amoxicillin/clavulanic acid, amoxicillin/sulbactam, piperacillin/tazobactam, aztreonam, ceftriaxone, cefotaxime, ceftazidime, ceftazidime/avibactam, cefepime, imipenem, meropenem, ciprofloxacin, trimethoprim/sulfamethoxazole, gentamicin, amikacin and tigecycline was determined using the Kirby-Bauer disc diffusion method. Detection of blaSCO-1 was established by Illumina WGS and bioinformatic analyses. All the publicly available genomes from K. pneumoniae carrying blaSCO-1 from the American continent were downloaded from the NCBI Isolates browser for assessment of phylogenetic comparisons.
Results: The isolate showed resistance to all the tested antibiotics except for ceftazidime/avibactam, carbapenems and tigecycline. Illumina WGS showed the presence of blaSCO-1 and blaOXA-1, blaSHV-28, blaTEM-1, blaCTX-M-15, as well as genes associated with resistance to quinolones, fosfomycin, aminoglycosides, sulphonamides, trimethoprim and phenicols. Moreover, the isolate was ST307, a high-risk clone of K. pneumoniae usually associated with blaCTX-M-15 and/or carbapenemases. Notwithstanding, the latter were not found on this isolate. Phylogenetic relationships were established by comparisons with 19 K. pneumoniae genomes carrying blaSCO-1 from other countries in the Americas, revealing at least three different clades.
Conclusions: This study highlights the importance of genomic surveillance of uncommon antimicrobial resistance genes such as blaSCO-1, which might contribute to further antimicrobial resistance levels in this country.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
