MicroRNA expression and their molecular targets in food allergies: a systematic review.

IF 5.7 2区医学 Q1 IMMUNOLOGY

Frontiers in Immunology Pub Date : 2025-05-12 eCollection Date: 2025-01-01 DOI:10.3389/fimmu.2025.1524392

Tekan Singh Rana, Rishipal Rastrapal Bansode, Jenny Pakhrin Rana, Leonard L Williams

{"title":"MicroRNA expression and their molecular targets in food allergies: a systematic review.","authors":"Tekan Singh Rana, Rishipal Rastrapal Bansode, Jenny Pakhrin Rana, Leonard L Williams","doi":"10.3389/fimmu.2025.1524392","DOIUrl":null,"url":null,"abstract":"Introduction: MicroRNAs (miRs) play an essential role in adaptive and innate immune systems by regulating the development of immune cells. However, detailed studies of miRs' role in food allergies are scarce compared to those of other allergic or non- allergic diseases. This systematic review aims to study miRs expression and its role in food allergies (FAs) and determine the signature miRs in FAs.Method: Research articles published since 2015 were selected from various databases: Scopus, PubMed, ScienceDirect, and Web of Science. Randomized clinical trials, observational clinical studies, and in vivo studies were assessed via the Cochrane Risk of Bias 2 tool, the Newcastle-Ottawa scale, and SYRCLE method, respectively. The characteristics of the included studies, population characteristics, and experimental details were extracted, and the data were synthesized narratively.Result: MiRs expression had been investigated in the context of cow milk allergy (CMA) and peanut allergy (PA) through both in vivo studies and clinical trials. Clinical trials included allergies to multiple combined foods, individual foods (such as milk, peanut, and what), and drugs and venom, while in vivo studies were conducted on milk, egg, and peanut allergies. MiR-146a, miR- 155, and miR-30a-5p were common miRs between in vivo studies and clinical trials. Moreover, few miRs were commonly studied between different types of food allergies. In clinical trials, miR-143-3p was studied in peanut allergy and non-celiac wheat sensitivity (NCWS), and miR-155 was studied in CMA and egg allergy in in vivo studies. Furthermore, the same miRs varied on their molecular target and effect depending on the type of food allergy.Discussion: The study on signature miRs and their molecular target determination for the therapeutic purpose of food allergy is in its initial stage. For individual food allergies, miRs determination via next-generation sequencing (NGS), their validation via polymerase chain reaction (PCR), and target molecule determination via RNA interference (RNAi) should be the focus of future studies in order to determine reliable signature miRs of food allergy.","PeriodicalId":12622,"journal":{"name":"Frontiers in Immunology","volume":"16 ","pages":"1524392"},"PeriodicalIF":5.7000,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104090/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fimmu.2025.1524392","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: MicroRNAs (miRs) play an essential role in adaptive and innate immune systems by regulating the development of immune cells. However, detailed studies of miRs' role in food allergies are scarce compared to those of other allergic or non- allergic diseases. This systematic review aims to study miRs expression and its role in food allergies (FAs) and determine the signature miRs in FAs.

Method: Research articles published since 2015 were selected from various databases: Scopus, PubMed, ScienceDirect, and Web of Science. Randomized clinical trials, observational clinical studies, and in vivo studies were assessed via the Cochrane Risk of Bias 2 tool, the Newcastle-Ottawa scale, and SYRCLE method, respectively. The characteristics of the included studies, population characteristics, and experimental details were extracted, and the data were synthesized narratively.

Result: MiRs expression had been investigated in the context of cow milk allergy (CMA) and peanut allergy (PA) through both in vivo studies and clinical trials. Clinical trials included allergies to multiple combined foods, individual foods (such as milk, peanut, and what), and drugs and venom, while in vivo studies were conducted on milk, egg, and peanut allergies. MiR-146a, miR- 155, and miR-30a-5p were common miRs between in vivo studies and clinical trials. Moreover, few miRs were commonly studied between different types of food allergies. In clinical trials, miR-143-3p was studied in peanut allergy and non-celiac wheat sensitivity (NCWS), and miR-155 was studied in CMA and egg allergy in in vivo studies. Furthermore, the same miRs varied on their molecular target and effect depending on the type of food allergy.

Discussion: The study on signature miRs and their molecular target determination for the therapeutic purpose of food allergy is in its initial stage. For individual food allergies, miRs determination via next-generation sequencing (NGS), their validation via polymerase chain reaction (PCR), and target molecule determination via RNA interference (RNAi) should be the focus of future studies in order to determine reliable signature miRs of food allergy.

查看原文本刊更多论文

食物过敏中MicroRNA表达及其分子靶点的系统综述。

MicroRNAs （miRs）通过调节免疫细胞的发育，在适应性免疫系统和先天免疫系统中发挥重要作用。然而，与其他过敏性或非过敏性疾病相比，miRs在食物过敏中的作用的详细研究很少。本综述旨在研究miRs在食物过敏（FAs）中的表达及其作用，并确定FAs中的特征miRs。方法：选取Scopus、PubMed、ScienceDirect、Web of Science等数据库中2015年以来发表的研究论文。随机临床试验、观察性临床研究和体内研究分别通过Cochrane风险偏倚2工具、纽卡斯尔-渥太华量表和sycle方法进行评估。提取纳入研究的特征、人群特征和实验细节，并对数据进行叙述性综合。结果：通过体内研究和临床试验研究了MiRs在牛奶过敏（CMA）和花生过敏（PA）背景下的表达。临床试验包括对多种组合食物过敏，对单一食物过敏（如牛奶，花生等），对药物和毒液过敏，而对牛奶，鸡蛋和花生过敏进行体内研究。miR- 146a、miR- 155和miR-30a-5p是体内研究和临床试验之间常见的miR。此外，很少有不同类型的食物过敏之间的mir被普遍研究。在临床试验中，研究了miR-143-3p在花生过敏和非乳糜泻小麦敏感（NCWS）中的作用，在体内研究了miR-155在CMA和鸡蛋过敏中的作用。此外，根据食物过敏的类型，相同的miRs在其分子靶点和作用上也有所不同。讨论：针对食物过敏治疗目的的特征miRs及其分子靶点的研究尚处于起步阶段。对于个体食物过敏，通过下一代测序（NGS）确定miRs，通过聚合酶链反应（PCR）验证miRs，以及通过RNA干扰（RNAi）确定靶分子应该是未来研究的重点，以便确定可靠的食物过敏特征miRs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Frontiers in Immunology IMMUNOLOGY-

CiteScore

9.80

自引率

11.00%

发文量

7153

审稿时长

14 weeks

期刊介绍： Frontiers in Immunology is a leading journal in its field, publishing rigorously peer-reviewed research across basic, translational and clinical immunology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Immunology is the official Journal of the International Union of Immunological Societies (IUIS). Encompassing the entire field of Immunology, this journal welcomes papers that investigate basic mechanisms of immune system development and function, with a particular emphasis given to the description of the clinical and immunological phenotype of human immune disorders, and on the definition of their molecular basis.