Lauric acid modulates cytochrome 4V2 expression in the human THP1 macrophages.

Q2 Pharmacology, Toxicology and Pharmaceutics

Drug metabolism and personalized therapy Pub Date : 2025-05-23 DOI:10.1515/dmpt-2025-0008

Yazun Bashir Jarrar, Wisam Nasser, Su-Jun Lee

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引用次数: 0

Abstract

Objectives: Macrophages play a major role in the inflammation. Recently, the expression of some cytochrome P450 (CYP450) 4 family enzymes was identified in the macrophages including CYP4V2, which metabolizes saturated fatty acids. Lauric acid is unsaturated fatty acid, which can induce inflammation. Its effect on the expression of CYP4V2 and the inflammatory mediators in macrophages is still unknown. This study aims to investigate the effect of lauric acid on the expression of CYP4V2 and cyclo-oxygenase 2 (COX2) in the human monocytes and macrophage THP1 cell line.

Methods: The THP1 monocyte cell line was differentiated into macrophages using 100 ng/mL PMA. Then, the cells were treated with 10 µM lauric acid for 72 h. The mRNA and protein expression of human CYP4V2 and COX2 were analyzed using real-time and western blot techniques, respectively.

Results: It was found that the mRNA and protein expression of CYP4V2 was upregulated after treatment of the macrophages with lauric acid in a dose-dependent manner. This upregulation was correlated with the expression of COX2.

Conclusions: It can be concluded from the results of this study that mRNA and protein expression of CYP4V2 are upregulated by lauric acid in correlation with the induction of inflammation. CYP4V2 can play a role in the inflammation process managed by macrophages.

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月桂酸调节人THP1巨噬细胞细胞色素4V2的表达。

目的：巨噬细胞在炎症中起主要作用。近年来，在巨噬细胞中发现了一些细胞色素P450 (CYP450) 4家族酶的表达，包括代谢饱和脂肪酸的CYP4V2。月桂酸是一种不饱和脂肪酸，可以引起炎症。其对巨噬细胞中CYP4V2和炎症介质表达的影响尚不清楚。本研究旨在探讨月桂酸对人单核细胞和巨噬细胞THP1细胞系中CYP4V2和环加氧酶2 （COX2）表达的影响。方法：用100 ng/mL PMA将THP1单核细胞分化为巨噬细胞。然后用10 µM月桂酸处理细胞72 h。采用real-time和western blot技术分别分析人CYP4V2和COX2 mRNA和蛋白的表达。结果：经月桂酸处理后，巨噬细胞CYP4V2 mRNA和蛋白表达呈剂量依赖性上调。这种上调与COX2的表达有关。结论：本研究结果表明月桂酸上调CYP4V2 mRNA和蛋白表达与炎症的诱导有关。CYP4V2可以在巨噬细胞控制的炎症过程中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug metabolism and personalized therapy Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

2.30

自引率

0.00%

发文量

期刊介绍： Drug Metabolism and Personalized Therapy (DMPT) is a peer-reviewed journal, and is abstracted/indexed in relevant major Abstracting Services. It provides up-to-date research articles, reviews and opinion papers in the wide field of drug metabolism research, covering established, new and potential drugs, environmentally toxic chemicals, the mechanisms by which drugs may interact with each other and with biological systems, and the pharmacological and toxicological consequences of these interactions and drug metabolism and excretion. Topics: drug metabolizing enzymes, pharmacogenetics and pharmacogenomics, biochemical pharmacology, molecular pathology, clinical pharmacology, pharmacokinetics and drug-drug interactions, immunopharmacology, neuropsychopharmacology.