Vitamin D metabolome in preterm infants: insights into postnatal metabolism.

IF 3.8 2区医学 Q1 MEDICAL LABORATORY TECHNOLOGY

Clinical chemistry and laboratory medicine Pub Date : 2025-05-22 DOI:10.1515/cclm-2025-0311

Tomas Matejek, Lukas Prchal, Bara Zapletalova, Veronika Pokorna, Jana Malakova, Vladimir Palicka, Ondrej Soukup

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引用次数: 0

Abstract

Objectives: To describe the structure of vitamin D metabolome and investigate the possible cause of high serum levels of C3 epimers of 25-(OH)D in preterm infants, we compared the vitamin D metabolites in umbilical cord blood with serum samples taken at 28 days of age.

Methods: We analysed 40 preterm infants (29+0-32+6 weeks of gestation). Cholecalciferol, 25-(OH)D, and its C3-epimers were measured using liquid chromatography. A microsomal study with human liver and kidney microsomes was conducted to assess vitamin D metabolism. Identified metabolites were then examined in cord blood and serum samples.

Results: Cholecalciferol, 25-(OH)D, and its C3-epimers were significantly lower in cord blood compared to serum at 28 days of age (p<0.001 for all metabolites). Conversely, metabolites from the microsomal study (monohydroxylated-, dihydroxylated-, and mono-oxylated dihydroxylated-cholecalciferol and their C3-epimers) were significantly higher in cord blood (p<0.001 for all).

Conclusions: Our findings indicate that cholecalciferol, 25-(OH)D, and its C3-epimers increase during the first month of life, suggesting functional biosynthesis and postnatal accumulation of these metabolites. Conversely, based on microsomal study results, it seems that biotransformation responsible for a degradation of vitamin D during the first month of life in preterm infants is functionally impaired.

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早产儿维生素D代谢组：对产后代谢的见解。

目的：为了描述维生素D代谢组的结构，探讨导致早产儿血清中25-(OH)D C3外显体水平升高的可能原因，我们比较了脐带血中维生素D代谢物与28日龄时的血清样本。方法：对40例早产儿（29+0 ~ 32+妊娠6周）进行分析。用液相色谱法测定胆钙化醇、25-(OH)D及其c3 -外显物。对人肝脏和肾脏微粒体进行了微粒体研究，以评估维生素D的代谢。然后在脐带血和血清样本中检测鉴定出的代谢物。结果：脐带血中胆骨化醇、25-(OH)D及其c3 -外显体在28日龄时明显低于血清。结论：我们的研究结果表明，在出生后的第一个月，胆骨化醇、25-(OH)D及其c3 -外显体增加，提示这些代谢物的功能性生物合成和出生后积累。相反，根据微粒体研究结果，在早产儿生命的第一个月，负责维生素D降解的生物转化似乎在功能上受损。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical chemistry and laboratory medicine 医学-医学实验技术

CiteScore

11.30

自引率

16.20%

发文量

306

审稿时长

3 months

期刊介绍： Clinical Chemistry and Laboratory Medicine (CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor over 3. CCLM is issued monthly, and it is published in print and electronically. CCLM is the official journal of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and publishes regularly EFLM recommendations and news. CCLM is the official journal of the National Societies from Austria (ÖGLMKC); Belgium (RBSLM); Germany (DGKL); Hungary (MLDT); Ireland (ACBI); Italy (SIBioC); Portugal (SPML); and Slovenia (SZKK); and it is affiliated to AACB (Australia) and SFBC (France). Topics: - clinical biochemistry - clinical genomics and molecular biology - clinical haematology and coagulation - clinical immunology and autoimmunity - clinical microbiology - drug monitoring and analysis - evaluation of diagnostic biomarkers - disease-oriented topics (cardiovascular disease, cancer diagnostics, diabetes) - new reagents, instrumentation and technologies - new methodologies - reference materials and methods - reference values and decision limits - quality and safety in laboratory medicine - translational laboratory medicine - clinical metrology Follow @cclm_degruyter on Twitter!