A simplified alternative to the conventional dialysis of the collagen extraction protocol

IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
AGDM Ampitiya , ST Gonapinuwala , CAN Fernando , MDST de Croos
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引用次数: 0

Abstract

One of the major disadvantages of the conventional fish collagen extraction protocol is its complicated, prolonged, and costly purification step which is associated with dialysis. Therefore, the focus of this study was to modify the purification step for a simple alternative and create a protocol to determine the efficiency of the alternative technique. Thus, Acid-soluble collagen was extracted from yellowfin tuna skin, and purification was carried out using both dialysis and a novel washing technique. The endpoint of this washing technique was determined using a refractometer and pH meter. Collagen yields from yellowfin tuna skin were 61.26 ± 0.63 % for the dialysis method and 62.03 ± 1.85 % for the washing technique. Collagens extracted by both methods were identified as Type I, exhibiting high purity and maintaining their triple helical structure. Under the naked eye, freeze-dried collagen appeared as a white sponge, while under scanning electron microscopy, it showed a porous, fibrous, and sheet-like structure. The washing technique proved more economically viable, time-efficient, and practical compared to dialysis. The endpoint determination approaches developed were straightforward and reliable. However, further scale-up studies are needed for both the washing technique and endpoint determination methods for its industrialisation.
一种简化的替代传统透析的胶原蛋白提取方案
传统的鱼胶原蛋白提取方案的主要缺点之一是其复杂,长时间和昂贵的纯化步骤,这与透析有关。因此,本研究的重点是将纯化步骤修改为简单的替代方法,并创建一个方案来确定替代技术的效率。因此,从黄鳍金枪鱼皮肤中提取酸溶性胶原蛋白,并采用透析和新型洗涤技术进行纯化。用折光计和pH计确定了这种洗涤技术的终点。透析法黄鳍金枪鱼皮肤胶原蛋白得率为61.26 ± 0.63 %,水洗法黄鳍金枪鱼皮肤胶原蛋白得率为62.03 ± 1.85 %。两种方法提取的胶原蛋白均为I型,纯度高,并保持其三螺旋结构。冻干的胶原蛋白肉眼呈白色海绵状,扫描电镜下呈多孔、纤维状、片状结构。事实证明,与透析相比,洗涤技术在经济上更可行、更省时、更实用。所开发的终点确定方法简单可靠。然而,需要对其工业化的洗涤技术和终点确定方法进行进一步的规模化研究。
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来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
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