AGDM Ampitiya , ST Gonapinuwala , CAN Fernando , MDST de Croos
{"title":"A simplified alternative to the conventional dialysis of the collagen extraction protocol","authors":"AGDM Ampitiya , ST Gonapinuwala , CAN Fernando , MDST de Croos","doi":"10.1016/j.fbp.2025.05.008","DOIUrl":null,"url":null,"abstract":"<div><div>One of the major disadvantages of the conventional fish collagen extraction protocol is its complicated, prolonged, and costly purification step which is associated with dialysis. Therefore, the focus of this study was to modify the purification step for a simple alternative and create a protocol to determine the efficiency of the alternative technique. Thus, Acid-soluble collagen was extracted from yellowfin tuna skin, and purification was carried out using both dialysis and a novel washing technique. The endpoint of this washing technique was determined using a refractometer and pH meter. Collagen yields from yellowfin tuna skin were 61.26 ± 0.63 % for the dialysis method and 62.03 ± 1.85 % for the washing technique. Collagens extracted by both methods were identified as Type I, exhibiting high purity and maintaining their triple helical structure. Under the naked eye, freeze-dried collagen appeared as a white sponge, while under scanning electron microscopy, it showed a porous, fibrous, and sheet-like structure. The washing technique proved more economically viable, time-efficient, and practical compared to dialysis. The endpoint determination approaches developed were straightforward and reliable. However, further scale-up studies are needed for both the washing technique and endpoint determination methods for its industrialisation.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"152 ","pages":"Pages 151-158"},"PeriodicalIF":3.5000,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioproducts Processing","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960308525000963","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
One of the major disadvantages of the conventional fish collagen extraction protocol is its complicated, prolonged, and costly purification step which is associated with dialysis. Therefore, the focus of this study was to modify the purification step for a simple alternative and create a protocol to determine the efficiency of the alternative technique. Thus, Acid-soluble collagen was extracted from yellowfin tuna skin, and purification was carried out using both dialysis and a novel washing technique. The endpoint of this washing technique was determined using a refractometer and pH meter. Collagen yields from yellowfin tuna skin were 61.26 ± 0.63 % for the dialysis method and 62.03 ± 1.85 % for the washing technique. Collagens extracted by both methods were identified as Type I, exhibiting high purity and maintaining their triple helical structure. Under the naked eye, freeze-dried collagen appeared as a white sponge, while under scanning electron microscopy, it showed a porous, fibrous, and sheet-like structure. The washing technique proved more economically viable, time-efficient, and practical compared to dialysis. The endpoint determination approaches developed were straightforward and reliable. However, further scale-up studies are needed for both the washing technique and endpoint determination methods for its industrialisation.
期刊介绍:
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.