Exploring UAV narrow-band hyperspectral indices and crop functional traits derived from radiative transfer models to detect wheat powdery mildew

Abstract

The wheat powdery mildew (WPM) is one of the most severe crop diseases worldwide, affecting wheat growth and causing yield losses. The WPM was a bottom-up disease that caused the loss of cell integrity, leaf wilting, and canopy structure damage with these symptoms altering the crop’s functional traits (CFT) and canopy spectra. The unmanned aerial vehicle (UAV)-based hyperspectral analysis became a mainstream method for WPM detection. However, the CFT changes experienced by infected wheats, the relationship between CFT and canopy spectra, and their role in WPM detection remained unclear, which might blur the understanding for the WPM infection. Therefore, this study aimed to propose a new method that considered the role of CFT for detecting WPM and estimating disease severity. The UAV hyperspectral data used in this study were collected from the Plant Protection Institute’s research demonstration base, Xinxiang city, China, covering a broad range of WPM severity (0–85 %) from 2022 to 2024. The potential of eight CFT [leaf structure parameter (N), leaf area index (LAI), chlorophyll a + b content (Cab), carotenoids (Car), Car/Cab, anthocyanins (Ant), canopy chlorophyll content (CCC) and average leaf angle (Deg)] obtained from a hybrid method combining a radiative transfer model and random forest (RF) and fifty-five narrow-band hyperspectral indices (NHI) was explored in WPM detection. Results indicated that N, Cab, Ant, Car, LAI, and CCC showed a decreasing trend with increasing disease severity, while Deg and Car/Cab exhibited the opposite pattern. There were marked differences between healthy samples and the two higher infection levels (moderate and severe infection) for Cab, Car, LAI, Deg, CCC, and Car/Cab. N and Ant only showed significant differences between the healthy samples and the highest infection level (severe infection). As Cab, Car, and Ant decreased, the spectral reflectance in the visible light region increased. The decrease in N and LAI was accompanied by a reduction in reflectance across the entire spectral range and the near-infrared area, which was exactly the opposite of Deg. The introduction of CFT greatly improved the accuracy of the WPM severity estimation model with R² of 0.92. Features related to photosynthesis, pigment content, and canopy structure played a decisive role in estimating WPM severity. Also, results found that the feature importance showed a remarkable interchange as increasing disease levels. Using features that described canopy structure changes, such as optimized soil adjusted vegetation index, LAI, visible atmospherically resistant indices, and CCC, the mild infection stage of this disease was most easily distinguished from healthy samples. In contrast, most severe impacts of WPM were best characterized by features related to photosynthesis (e.g., photochemical reflectance index 515) and pigment content (e.g., normalized phaeophytinization index). This study help deepen the understanding of symptoms and spectral responses caused by WPM infection.