Combinatorial characterization of bacterial taxa-driven differences in the microbiome of oyster beds.

Sustainable microbiology Pub Date : 2025-05-05 eCollection Date: 2025-01-01 DOI:10.1093/sumbio/qvaf006
Erika L Cyphert, Sanjiev Nand, Gabriela Franco, Michael Hajkowski, Luzmaria Soto, Danica Marvi Lee, Matthew C Ferner, Chela J Zabin, Jeffrey Blumenthal, Anna K Deck, Katharyn E Boyer, Laura W Burrus, Christopher J Hernandez, Archana Anand
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Abstract

Oyster reefs and beds provide crucial ecosystem services, including water filtration, coastal protection, and habitat provision for marine species. However, these habitats face significant threats from climate change and anthropogenic stressors. To address these challenges, numerous oyster restoration initiatives have been undertaken globally. Intertidal microbial communities, comprising diverse bacteria, archaea, and unicellular eukaryotes, drive key biogeochemical processes, but remain poorly understood in ecosystems targeted for oyster restoration. We investigated the microbiome associated with the Olympia oyster (Ostrea lurida), a key restoration target in the western USA, by characterizing microbial abundance and diversity in sediment, oyster-associated biofilm, and oysters at four sites in San Francisco Bay. Our findings revealed distinct microbial assemblages in oysters and sediment compared to biofilm. Diversity, measured by the Shannon index, was highest at Heron's Head (5.47), followed by Brickyard Park (5.35), Dunphy Park (5.17), and Point Pinole (4.85). Notably, microbes Ruminococcus, Streptococcus, Staphylococcus, Prevotella, Porphyromonas, Parvimonas, Neisseria, Lactococcus, Haemophilus, Fusobacterium, Dorea, Clostridium, Campylobacter, Bacteroides, and Akkermansia were positively associated with biofilm collected from hard surfaces where oysters were attached at most sites. Potential implications of these microbial associations for oyster growth, survival, and interactions with environmental factors remain largely unexplored. Future research is warranted on culturing specific microbes to assess their functions and investigating environmental and ecological drivers of microbial diversity to determine relationships indicative of oyster health and potential for enhancing oyster recruitment.

牡蛎床微生物组细菌分类驱动差异的组合表征。
牡蛎礁和牡蛎床提供重要的生态系统服务,包括水过滤、海岸保护和为海洋物种提供栖息地。然而,这些栖息地面临着气候变化和人为压力的重大威胁。为了应对这些挑战,全球开展了许多牡蛎恢复计划。潮间带微生物群落,包括多种细菌,古细菌和单细胞真核生物,驱动关键的生物地球化学过程,但在牡蛎恢复的生态系统中仍然知之甚少。通过表征旧金山湾四个地点沉积物、牡蛎相关生物膜和牡蛎中的微生物丰度和多样性,研究了与美国西部重要恢复目标奥林匹亚牡蛎(Ostrea lurida)相关的微生物组。与生物膜相比,我们的发现揭示了牡蛎和沉积物中不同的微生物组合。以Shannon指数衡量的多样性以Heron's Head最高(5.47),其次是Brickyard Park(5.35)、Dunphy Park(5.17)和Point Pinole(4.85)。值得注意的是,Ruminococcus、Streptococcus、Staphylococcus、Prevotella、Porphyromonas、Parvimonas、Neisseria、Lactococcus、Haemophilus、Fusobacterium、Dorea、Clostridium、Campylobacter、Bacteroides和Akkermansia等微生物与从坚硬表面收集的生物膜呈正相关,而坚硬表面的大多数地方都附着着牡蛎。这些微生物对牡蛎生长、存活和与环境因素的相互作用的潜在影响在很大程度上仍未被探索。未来的研究需要培养特定的微生物来评估它们的功能,并调查微生物多样性的环境和生态驱动因素,以确定指示牡蛎健康的关系和增强牡蛎招募的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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