Zizheng Suo , Bocheng Pan , Hailong Shi , Linhui Ma , Yuxiang Zheng , Wenjie Xu , Lina Lin , Enze Zhang , Lijuan Wang , Mingzhu Zhang , Yinyin Qu , Hui Zheng , Xingyu Gao , Cheng Ni
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引用次数: 0
Abstract
Aims
The rapidly growing scale and complexity of single-cell transcriptomic data in brain research make it increasingly difficult for traditional methods to extract meaningful insights efficiently, highlighting the need for artificial intelligence.
Materials and methods
We presented the Hybrid Learning-based Brain single-cell Prediction Framework (HL-BscPF), designed to automate cell type classification and reveal disease-related pathways in the brain. HL-BscPF integrates ItClust and TOSICA models, combining autoencoder-based dimensionality reduction with transformer architecture to enhance predictive accuracy. HL-BscPF was evaluated using brain scRNA-seq datasets representing various neuropathological states, and its predictive performance was benchmarked against ground-truth annotations.
Key findings
Applied to four brain-specific single-cell datasets, including aging, Alzheimer's disease, postoperative cognitive dysfunction, and stroke, HL-BscPF accurately classified cell types and uncovered key functional alterations in neuronal and glial populations. TOSICA showed higher accuracy in large-scale datasets due to its multi-head self-attention capabilities, whereas ItClust performed optimally in cases with lower cell diversity, demonstrating their complementary strengths. By providing precise cell identification and novel insights into brain-specific pathway dysregulation, HL-BscPF offers a powerful tool for extracting meaningful insights from vast single-cell datasets, enabling a deeper understanding of the complex neuropathologies.
Significance
HL-BscPF demonstrates exceptional accuracy and interpretability in cell type annotation and functional analysis, uncovering critical disease-related mechanisms. This framework offers a powerful tool for advancing single-cell research in brain pathologies.
期刊介绍:
Life Sciences is an international journal publishing articles that emphasize the molecular, cellular, and functional basis of therapy. The journal emphasizes the understanding of mechanism that is relevant to all aspects of human disease and translation to patients. All articles are rigorously reviewed.
The Journal favors publication of full-length papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research. The Journal welcomes reviews on topics of wide interest to investigators in the life sciences. We particularly encourage submission of brief, focused reviews containing high-quality artwork and require the use of mechanistic summary diagrams.