Mechanistic study of the association between microRNA-126 and hypertension in obstructive sleep apnea-hypopnea syndrome.

IF 3.5 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE
Xiaobo Huang, Wenjuan Hu, Lijun Chen, Liting Ma, Gengen Yu, Juanxia Chen, Huifang Zhang, Fan Cao, Huijie Wang
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引用次数: 0

Abstract

Objective: This study explored miRNA-126 and HIF-1α levels in patients with OSAHS-related hypertension. To analyze the relationship between miRNA-126 and OSAHS-related hypertension and explore the pathogenic mechanisms of miRNA-126 and the HIF-1α pathway.

Materials and methods: The 120 participants were assigned to four groups based on their apnea-hypopnea index (AHI) and the presence of hypertension: healthy control group, hypertension group, non-hypertensive OSAHS group, and OSAHS-related hypertension group, with 30 objects in each group. All subjects underwent overnight sleep monitoring and 24-hour (h) ambulatory blood pressure. Their blood samples were analyzed for the following parameters: triglycerides (TG), fasting blood glucose (GLU), total cholesterol (TC), HDL, uric acid (UA), and LDL, hypoxia-inducible factor-1 alpha (HIF-1α), VEGF, C-reactive protein (CRP), IL-6 and tumor necrosis factor alpha (TNF-α). The relative expression levels of miRNA-126 were assessed by RT-qPCR and Western blotting, and dual-luciferase reporter assays were conducted to verify the association between miR-126-3p and HIF1-α.

Results: Results indicated that HIF-1α is a target gene of miRNA-126 and is negatively regulated by miRNA-126; Levels of miRNA-126 were significantly lower in OSAHS and hypertension patients relative to healthy controls, with the lowest levels observed in the OSAHS-related hypertension group(p < .05). miRNA-126 levels were negatively correlated with HIF-1α, VEGF, TNF-α, CRP, IL-6, TG, TC, LDL, GLU and UA levels (p < .01), while it showed positive correlation with HDL level (p < .01).

Conclusion: The level of miRNA-126 in patients with OSAHS-related hypertension is lower than that in patients with OSAHS alone and those with hypertension alone.

microRNA-126与阻塞性睡眠呼吸暂停低通气综合征高血压相关的机制研究。
目的:探讨osahs相关性高血压患者的miRNA-126和HIF-1α水平。分析miRNA-126与osahs相关性高血压的关系,探讨miRNA-126与HIF-1α通路的致病机制。材料与方法:120名受试者根据其呼吸暂停低通气指数(AHI)和是否存在高血压分为健康对照组、高血压组、非高血压性OSAHS组和OSAHS相关高血压组,每组30名。所有受试者都进行了夜间睡眠监测和24小时动态血压监测。对其血液样本进行以下参数分析:甘油三酯(TG)、空腹血糖(GLU)、总胆固醇(TC)、高密度脂蛋白(HDL)、尿酸(UA)、低密度脂蛋白(LDL)、缺氧诱导因子-1α (HIF-1α)、VEGF、c反应蛋白(CRP)、IL-6和肿瘤坏死因子α (TNF-α)。RT-qPCR和Western blotting检测miRNA-126的相对表达水平,采用双荧光素酶报告基因检测验证miR-126-3p与HIF1-α的相关性。结果:结果表明HIF-1α是miRNA-126的靶基因,受miRNA-126的负调控;miRNA-126在OSAHS和高血压患者中均显著低于健康对照组,其中OSAHS相关高血压组miRNA-126水平最低(p p p)。结论:OSAHS相关高血压患者miRNA-126水平低于单纯OSAHS患者和单纯高血压患者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.90
自引率
0.80%
发文量
66
审稿时长
6-12 weeks
期刊介绍: Clinical and Experimental Hypertension is a reputable journal that has converted to a full Open Access format starting from Volume 45 in 2023. While previous volumes are still accessible through a Pay to Read model, the journal now provides free and open access to its content. It serves as an international platform for the exchange of up-to-date scientific and clinical information concerning both human and animal hypertension. The journal publishes a wide range of articles, including full research papers, solicited and unsolicited reviews, and commentaries. Through these publications, the journal aims to enhance current understanding and support the timely detection, management, control, and prevention of hypertension-related conditions. One notable aspect of Clinical and Experimental Hypertension is its coverage of special issues that focus on the proceedings of symposia dedicated to hypertension research. This feature allows researchers and clinicians to delve deeper into the latest advancements in this field. The journal is abstracted and indexed in several renowned databases, including Pharmacoeconomics and Outcomes News (Online), Reactions Weekly (Online), CABI, EBSCOhost, Elsevier BV, International Atomic Energy Agency, and the National Library of Medicine, among others. These affiliations ensure that the journal's content receives broad visibility and facilitates its discoverability by professionals and researchers in related disciplines.
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