Nanodiscs and solution nuclear magnetic resonance

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-05-26 DOI:10.1016/j.sbi.2025.103067

Olga Vinogradova

引用次数: 0

Abstract

Although membrane proteins constitute a significant portion of the genomes of all species and represent well-validated targets for numerous therapeutic interventions, high-resolution structural knowledge of this class of proteins still falls behind that of their soluble counterparts. Despite serious technological developments in the methods presently available for structural characterizations, as well as decades spent on such investigations, membrane proteins remain notoriously difficult to study. This is particularly true for environments which mimic native membranes well enough to maintain their proper functional states. This mini review covers the most recent advances in the structural and dynamic characterization of membrane proteins through the utilization of solution nuclear magnetic resonance methods applied to lipid nanodiscs.

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纳米片和溶液核磁共振

尽管膜蛋白构成了所有物种基因组的重要部分，并代表了许多治疗干预措施的良好验证靶点，但这类蛋白的高分辨率结构知识仍然落后于其可溶性对立物。尽管目前用于结构表征的方法有了重大的技术发展，并且在这类研究上花费了数十年的时间，但膜蛋白的研究仍然是出了名的困难。这对于那些能够很好地模拟天然膜以维持其正常功能状态的环境来说尤其如此。这篇综述涵盖了通过应用于脂质纳米盘的溶液核磁共振方法在膜蛋白结构和动态表征方面的最新进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation