Quantification of mechanical-cytoskeletal coupling in human corneal cells across myopia severity.

IF 2.4 Q3 BIOPHYSICS
Geng Junyuan, Lu Yue, Li Shuangcheng, Wang Yan, Zhao Xin
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引用次数: 0

Abstract

Myopia is a prevalent refractive eye disorder closely associated with alterations in corneal biomechanical properties. As fundamental units of corneal tissue, corneal cells significantly influence myopia progression through their nanomechanical characteristics. However, the biophysical mechanisms underlying this process, particularly in human corneal cells, remain unclear. This study investigates the coupling between mechanical properties and cytoskeletal morphology in human corneal cells across varying myopia severity levels. Utilizing atomic force microscopy (AFM), the Young's modulus and adhesion properties of corneal cells obtained from patients with low, moderate, and high myopia were assessed. Additionally, the cytoskeletal morphological variations were quantified by calculating the fractal dimension from AFM topography images. Experimental results reveal that with increasing myopia severity, corneal cells exhibit decreased stiffness, increased adhesion, and reduced regularity and stability of the cytoskeletal network. This evidence highlights a coupling relationship between biomechanical properties and cytoskeletal morphology in human corneal cells during myopia development at the cellular scale, offering significant insights into the pathogenesis of myopia and potential avenues for innovative preventive strategies. VIDEO ABSTRACT.

人角膜细胞机械-细胞骨架偶联在近视严重程度上的定量分析。
近视是一种常见的屈光性眼病,与角膜生物力学特性的改变密切相关。作为角膜组织的基本单位,角膜细胞通过其纳米力学特性显著影响近视的进展。然而,这一过程背后的生物物理机制,特别是在人类角膜细胞中,仍不清楚。本研究探讨了不同近视严重程度的人角膜细胞的力学特性和细胞骨架形态之间的耦合。利用原子力显微镜(AFM)对低、中、高度近视患者角膜细胞的杨氏模量和粘附特性进行了评估。此外,通过计算AFM形貌图像的分形维数来量化细胞骨架形态的变化。实验结果表明,随着近视严重程度的增加,角膜细胞的硬度降低,粘附性增加,细胞骨架网络的规律性和稳定性降低。这些证据在细胞尺度上强调了近视发展过程中人类角膜细胞生物力学特性和细胞骨架形态之间的耦合关系,为近视的发病机制和创新预防策略的潜在途径提供了重要的见解。
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来源期刊
Biophysical reports
Biophysical reports Biophysics
CiteScore
2.40
自引率
0.00%
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0
审稿时长
75 days
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