Fentanyl-Induced Transformations in Composition of Lipid Droplets in Central Nervous System Cells revealed by Ramanomics.

IF 5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Rahul K Das, Andrey N Kuzmin, Artem Pliss, Supriya D Mahajan, Shobha Shukla, Paras N Prasad
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引用次数: 0

Abstract

Quantitative characterization of the transformations of subcellular molecular environment in response to fentanyl exposure in human microglia and astrocytes is warranted to provide insight into the regulation of neuroinflammatory responses and neural survival in the scenario of opiate drug addiction. Cytoplasmic lipid droplets (LD) act as depot for exogeneous hydrophobic molecules, such as fentanyl, which can lead to increased drug accumulation and alteration of their metabolism. In the present work, we have used an emerging Ramanomics technique that combines quantitative micro-Raman spectrometry with biomolecular component analysis to unravel fentanyl induced changes in concentrations of phospholipids, sterols, glycogen, sphingomyelin, phosphocholine as well as RNA and proteins, in the LDs of microglia and astrocytes. To our knowledge, this study represents, the first report on the effect of fentanyl overdose in the biomolecular composition of distinct organelles in single live cells, towards therapeutics advances to treat fentanyl addiction and development of diagnostic assays of illicit opiate abuse.

芬太尼诱导中枢神经系统细胞脂滴组成的转变
定量表征人类小胶质细胞和星形胶质细胞暴露于芬太尼后亚细胞分子环境的变化,有助于深入了解阿片类药物成瘾情景下神经炎症反应和神经存活的调节。细胞质脂滴(LD)作为外源疏水分子(如芬太尼)的仓库,可导致药物积累增加和代谢改变。在目前的工作中,我们使用了一种新兴的Ramanomics技术,该技术将定量微拉曼光谱法与生物分子成分分析相结合,以揭示芬太尼在小胶质细胞和星形胶质细胞的LDs中诱导的磷脂、甾醇、糖原、鞘磷脂、胆碱以及RNA和蛋白质浓度的变化。据我们所知,这项研究代表了第一份关于芬太尼过量对单个活细胞中不同细胞器生物分子组成影响的报告,对芬太尼成瘾治疗的治疗进展和非法阿片类药物滥用诊断分析的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Lipid Research
Journal of Lipid Research 生物-生化与分子生物学
CiteScore
11.10
自引率
4.60%
发文量
146
审稿时长
41 days
期刊介绍: The Journal of Lipid Research (JLR) publishes original articles and reviews in the broadly defined area of biological lipids. We encourage the submission of manuscripts relating to lipids, including those addressing problems in biochemistry, molecular biology, structural biology, cell biology, genetics, molecular medicine, clinical medicine and metabolism. Major criteria for acceptance of articles are new insights into mechanisms of lipid function and metabolism and/or genes regulating lipid metabolism along with sound primary experimental data. Interpretation of the data is the authors’ responsibility, and speculation should be labeled as such. Manuscripts that provide new ways of purifying, identifying and quantifying lipids are invited for the Methods section of the Journal. JLR encourages contributions from investigators in all countries, but articles must be submitted in clear and concise English.
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