Comprehensive analysis of Brassica napus Glycosyl hydrolase family 1 genes and functional analysis of BnaBGLU27.C08 in clubroot resistance.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BMC Genomics Pub Date : 2025-05-21 DOI:10.1186/s12864-025-11638-2

Yiji Shi, Xiang Liu, Shunjun Bao, Ye Li, Yuqing Shi, Chen Liang, Hongyu Sha, Zihao Yu, Qing Su, Xiyu Zhang, Xiaoyan Li, Weinan Sun, Chengyu Yu, Keqi Li, Zhen Huang

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引用次数: 0

Abstract

Background: β-Glycosidases (BGLUs), belonging to the Glycoside Hydrolase Family 1 (GH1), play crucial roles in plant growth, development, and stress adaptation. While BGLUs have been extensively characterized in various plants, a comprehensive analysis of the BGLU family in Brassica napus has yet to be conducted.

Results: In this study, we identified 131 BnaBGLU genes in B. napus and classified them into 11 subfamilies. Gene duplication analysis revealed that the expansion of the BnaBGLU family primarily resulted from segmental duplications. Structural analysis revealed high conservation within subfamilies, while promoter cis-element profiling and transcriptional studies under phytohormone treatments and abiotic stresses demonstrated BnaBGLUs respond to diverse environmental stimuli. Additionally, BnaBGLUs exhibit tissue-specific expression patterns. Notably, BnaBGLU27.C08 was significantly upregulated 96 h post-inoculation with Plasmodiophora brassicae in the root of clubroot-resistant (CR) B. napus varieties. Furthermore, overexpression of BnaBGLU27.C08 in Arabidopsis significantly enhanced resistance to clubroot disease.

Conclusions: These findings establish a foundation for further investigation on the BnaBGLU family in B. napus and offer potential candidate genes for breeding CR B. napus varieties.

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甘蓝型油菜糖基水解酶家族1基因综合分析及BnaBGLU27的功能分析。C08在根瘤菌抗性中。

背景：β-糖苷酶（BGLUs）属于糖苷水解酶家族1 (GH1)，在植物生长发育和逆境适应中起着至关重要的作用。虽然BGLU已在多种植物中被广泛表征，但对甘蓝型油菜BGLU家族的全面分析尚未开展。结果：从甘蓝型油菜中鉴定出131个BnaBGLU基因，并将其划分为11个亚科。基因重复分析表明，BnaBGLU家族的扩增主要是由片段重复引起的。结构分析显示，BnaBGLUs在亚家族中具有高度的保守性，而启动子顺式元件分析和植物激素处理和非生物胁迫下的转录研究表明，BnaBGLUs对多种环境刺激有响应。此外，BnaBGLUs表现出组织特异性表达模式。值得注意的是,BnaBGLU27。C08在抗棒孢霉（CR）甘蓝型油菜（B. napus）根内接种后96 h显著上调。此外，BnaBGLU27过表达。拟南芥中C08显著增强了对棍棒病的抗性。结论：这些发现为进一步研究甘蓝型油菜BnaBGLU家族奠定了基础，并为甘蓝型油菜品种的选育提供了潜在的候选基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.