Protective Effects of Pleurotus Species on UVB-Induced Skin Disorders at Clinically Relevant Plasma Concentrations of the Antioxidant Ergothioneine in Hairless Mice.

IF 1.7 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Motoki Hanayama, Takahiro Ishimoto, Akira Moritomo, Reiya Yamashita, Junya Kawai, Koichiro Mori, Yukio Kato
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Abstract

Ergothioneine (ERGO) has antioxidant and anti-inflammatory activities in UV-irradiated skin cells in vitro; however, there is no evidence about the effects of dietary ERGO on UV-induced skin damage or ERGO skin distribution in vivo. This study examined the protective effects of ERGO-rich edible mushrooms Pleurotus species against UVB-induced skin damage and the exposure to ERGO in the plasma and skin. Hos : HR-1 hairless mice were fed with or without freeze-dried cross-bred Pleurotus species (PS) or Pleurotus eringii (PE) and were exposed to UVB. Dietary intake of PS or PE significantly alleviated UVB-induced reductions in skin moisture content, increases in transepidermal water loss and oxidative stress markers, and epidermal thickening at plasma ERGO concentrations of 30-40 μM. Additionally, ingestion of PS significantly suppressed UVB-induced expression of pro-inflammatory cytokines. These results suggest that ingesting PS and PE may protect against UVB-induced skin disorders through antioxidant and anti-inflammatory activities at clinically relevant ERGO concentrations. Ingestion of PS and PE led to an increase in epidermal ERGO concentration to levels that were approx. 100 times higher than the ERGO concentration required for significant suppression of UVB-induced intracellular reactive oxygen species in immortalized human keratinocyte HaCaT cells. This suggests that the beneficial effects of PS and PE may be at least partly due to the antioxidant effects of ERGO in murine skin. Overall, ingestion of ERGO-rich Pleurotus species resulted in efficient distribution of ERGO to the skin and protective effects against UVB-induced skin damage, suggesting that these mushrooms may have beneficial effects in humans.

在无毛小鼠抗氧化剂麦角硫因临床相关血浆浓度下,侧耳菌对uvb诱导的皮肤病的保护作用。
麦角硫因(ERGO)在体外紫外线照射皮肤细胞中具有抗氧化和抗炎活性然而,没有证据表明膳食中的ERGO对紫外线引起的皮肤损伤或ERGO在体内的皮肤分布有影响。本研究考察了富含ERGO的平菇(Pleurotus)对uvb引起的皮肤损伤以及血浆和皮肤暴露于ERGO的保护作用。实验采用冻干杂交侧耳菇(PS)或侧耳菇(PE)分别饲喂或不饲喂HR-1型无毛小鼠,并对其进行UVB照射。在血浆ERGO浓度为30-40 μM时,膳食摄入PS或PE可显著缓解uvb诱导的皮肤含水量降低、经皮失水和氧化应激标志物增加以及表皮增厚。此外,摄入PS可显著抑制uvb诱导的促炎细胞因子的表达。这些结果表明,在临床相关的ERGO浓度下,摄入PS和PE可能通过抗氧化和抗炎活性来预防uvb诱导的皮肤疾病。摄入PS和PE导致表皮ERGO浓度增加到大约的水平。比ERGO浓度高100倍,显著抑制uvb诱导的永生化人角质细胞HaCaT细胞内活性氧。这表明,PS和PE的有益作用可能至少部分是由于ERGO对小鼠皮肤的抗氧化作用。总的来说,摄入富含ERGO的侧耳菇可以有效地将ERGO分配到皮肤,并对uvb引起的皮肤损伤具有保护作用,这表明这些蘑菇可能对人类有益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.50
自引率
5.00%
发文量
247
审稿时长
2 months
期刊介绍: Biological and Pharmaceutical Bulletin (Biol. Pharm. Bull.) began publication in 1978 as the Journal of Pharmacobio-Dynamics. It covers various biological topics in the pharmaceutical and health sciences. A fourth Society journal, the Journal of Health Science, was merged with Biol. Pharm. Bull. in 2012. The main aim of the Society’s journals is to advance the pharmaceutical sciences with research reports, information exchange, and high-quality discussion. The average review time for articles submitted to the journals is around one month for first decision. The complete texts of all of the Society’s journals can be freely accessed through J-STAGE. The Society’s editorial committee hopes that the content of its journals will be useful to your research, and also invites you to submit your own work to the journals.
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