Rapid review: Recent advances in in vitro models for the study of Cryptosporidium parvum

IF 1.7 Q3 PARASITOLOGY
Mathilde S. Varegg, Ian D. Woolsey, Lucy J. Robertson, Alejandro Jiménez-Meléndez
{"title":"Rapid review: Recent advances in in vitro models for the study of Cryptosporidium parvum","authors":"Mathilde S. Varegg,&nbsp;Ian D. Woolsey,&nbsp;Lucy J. Robertson,&nbsp;Alejandro Jiménez-Meléndez","doi":"10.1016/j.crpvbd.2025.100269","DOIUrl":null,"url":null,"abstract":"<div><div><em>Cryptosporidium</em> research has been hampered by the lack of <em>in vitro</em> models that can recapitulate the life cycle of the parasite, thus relying on repeated animal infections. Traditional <em>in vitro</em> systems, employing cancerous cell lines, have been unable to support sexual reproduction, but have been widely employed for drug screening assays and allowed transcriptome mapping of the parasite, but extrapolation of those results to <em>in vivo</em> infections is limited. In recent years, intestinal organoids (enteroids), grown as 3D structures, have come to be recognized as more physiologically relevant, complex systems, since they more accurately reproduce the cell populations present in the small intestine. A key advantage of these systems is their ability to fulfil the life cycle of the parasite. However, studies employing bovine organoids, the target species of the major zoonotic species <em>Cryptosporidium parvum</em>, are lacking. Future research should emphasize bioengineered systems, with heterogeneous populations of intestinal epithelial and mesenchymal cells, to advance the <em>in vitro</em> field closer to <em>in vivo</em> infection models. The present review summarizes the history of cell line use in <em>Cryptosporidium</em> research and the most recent advances in organoids, bio-engineered and organ-on-a-chip platforms, including methodological approaches used to facilitate exposure of the apical side of the target cells to the parasite, and the influence of mechanical forces and microenvironment.</div></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"7 ","pages":"Article 100269"},"PeriodicalIF":1.7000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current research in parasitology & vector-borne diseases","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667114X25000299","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Cryptosporidium research has been hampered by the lack of in vitro models that can recapitulate the life cycle of the parasite, thus relying on repeated animal infections. Traditional in vitro systems, employing cancerous cell lines, have been unable to support sexual reproduction, but have been widely employed for drug screening assays and allowed transcriptome mapping of the parasite, but extrapolation of those results to in vivo infections is limited. In recent years, intestinal organoids (enteroids), grown as 3D structures, have come to be recognized as more physiologically relevant, complex systems, since they more accurately reproduce the cell populations present in the small intestine. A key advantage of these systems is their ability to fulfil the life cycle of the parasite. However, studies employing bovine organoids, the target species of the major zoonotic species Cryptosporidium parvum, are lacking. Future research should emphasize bioengineered systems, with heterogeneous populations of intestinal epithelial and mesenchymal cells, to advance the in vitro field closer to in vivo infection models. The present review summarizes the history of cell line use in Cryptosporidium research and the most recent advances in organoids, bio-engineered and organ-on-a-chip platforms, including methodological approaches used to facilitate exposure of the apical side of the target cells to the parasite, and the influence of mechanical forces and microenvironment.
快速回顾:小隐孢子虫体外模型研究的最新进展
隐孢子虫的研究一直受到阻碍,因为缺乏能够概括寄生虫生命周期的体外模型,因此依赖于反复的动物感染。使用癌细胞系的传统体外系统无法支持有性繁殖,但已广泛用于药物筛选试验并允许寄生虫的转录组图谱绘制,但将这些结果外推到体内感染是有限的。近年来,以3D结构生长的肠道类器官(enteroid)被认为是生理上更相关的复杂系统,因为它们更准确地再现了小肠中的细胞群。这些系统的一个关键优势是它们能够完成寄生虫的生命周期。然而,利用牛类器官作为主要人畜共患物种小隐孢子虫的靶种的研究是缺乏的。未来的研究应强调具有异质肠道上皮细胞和间充质细胞群体的生物工程系统,使体外领域更接近体内感染模型。本文综述了隐孢子虫细胞系研究的历史以及类器官、生物工程和器官芯片平台的最新进展,包括用于促进靶细胞顶侧暴露于寄生虫的方法方法,以及机械力和微环境的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
3.60
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信