Ekaterina I Smirnova, Victoria A Shestakova, Elena V Isaeva, Anastas A Kisel, Elena M Yatsenko, Peter V Shegay, Andrey D Kaprin, Ilya D Klabukov, Denis S Baranovskii
{"title":"Isolation and Culturing of Newborn Rat Nasal Chondrocytes.","authors":"Ekaterina I Smirnova, Victoria A Shestakova, Elena V Isaeva, Anastas A Kisel, Elena M Yatsenko, Peter V Shegay, Andrey D Kaprin, Ilya D Klabukov, Denis S Baranovskii","doi":"10.1007/7651_2025_632","DOIUrl":null,"url":null,"abstract":"<p><p>Chondrocyte-based strategies are widely used for cartilage repair and show promising results in the treatment of articular cartilage damage and osteoarthritis. In cartilage repair approaches that involve the implantation of autologous chondrocytes or tissue-engineered scaffolds, it is essential to obtain significant amounts of viable cells. Nasal chondrocytes serve as a clinically valuable source of cells for cartilage regeneration, and their isolation is a critical step in cartilage tissue engineering. This chapter outlines an optimized protocol for obtaining nasal chondrocytes from neonatal rats using a Leica VT1200 vibratome to obtain nasal cartilage fragments. Enzymatic isolation of nasal chondrocytes and their subsequent in vitro culture highlight the importance of the stem and progenitor cell niche in supporting the further culture and differentiation of these cells for effective cartilage regeneration.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/7651_2025_632","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Chondrocyte-based strategies are widely used for cartilage repair and show promising results in the treatment of articular cartilage damage and osteoarthritis. In cartilage repair approaches that involve the implantation of autologous chondrocytes or tissue-engineered scaffolds, it is essential to obtain significant amounts of viable cells. Nasal chondrocytes serve as a clinically valuable source of cells for cartilage regeneration, and their isolation is a critical step in cartilage tissue engineering. This chapter outlines an optimized protocol for obtaining nasal chondrocytes from neonatal rats using a Leica VT1200 vibratome to obtain nasal cartilage fragments. Enzymatic isolation of nasal chondrocytes and their subsequent in vitro culture highlight the importance of the stem and progenitor cell niche in supporting the further culture and differentiation of these cells for effective cartilage regeneration.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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