Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry

IF 6.6 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Science Signaling Pub Date : 2025-05-20 DOI:10.1126/scisignal.adw1245

Nils Helge Schebb, Nadja Kampschulte, Gerhard Hagn, Kathrin Plitzko, Sven W. Meckelmann, Soumita Ghosh, Robin Joshi, Julia Kuligowski, Dajana Vuckovic, Marina T. Botana, Ángel Sánchez-Illana, Fereshteh Zandkarimi, Aditi Das, Jun Yang, Louis Schmidt, Antonio Checa, Helen M. Roche, Aaron M. Armando, Matthew L. Edin, Fred B. Lih, Juan J. Aristizabal-Henao, Sayuri Miyamoto, Francesca Giuffrida, Arieh Moussaieff, Rosário Domingues, Michael Rothe, Christine Hinz, Ujjalkumar Subhash Das, Katharina M. Rund, Ameer Y. Taha, Robert K. Hofstetter, Markus Werner, Oliver Werz, Astrid S. Kahnt, Justine Bertrand-Michel, Pauline Le Faouder, Robert Gurke, Dominique Thomas, Federico Torta, Ivana Milic, Irundika H. K. Dias, Corinne M. Spickett, Denise Biagini, Tommaso Lomonaco, Helena Idborg, Jun-Yan Liu, Maria Fedorova, David A. Ford, Anne Barden, Trevor A. Mori, Paul D. Kennedy, Kirk Maxey, Julijana Ivanisevic, Hector Gallart-Ayala, Cécile Gladine, Markus Wenk, Jean-Marie Galano, Thierry Durand, Ken D. Stark, Coral Barbas, Ulrike Garscha, Stacy L. Gelhaus, Uta Ceglarek, Nicolas Flamand, Julian L. Griffin, Robert Ahrends, Makoto Arita, Darryl C. Zeldin, Francisco J. Schopfer, Oswald Quehenberger, Randall Julian, Anna Nicolaou, Ian A. Blair, Michael P. Murphy, Bruce D. Hammock, Bruce Freeman, Gerhard Liebisch, Charles N. Serhan, Harald C. Köfeler, Per-Johan Jakobsson, Dieter Steinhilber, Michael H. Gelb, Michal Holčapek, Ruth Andrew, Martin Giera, Garret A. FitzGerald, Robert C. Murphy, John W. Newman, Edward A. Dennis, Kim Ekroos, Ginger L. Milne, Miguel A. Gijón, Hubert W. Vesper, Craig E. Wheelock, Valerie B. O’Donnell

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引用次数: 0

Abstract

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography–mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

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液相色谱-质谱法分析氧脂类的技术建议

几种氧磷脂是有效的脂质介质，调节健康和疾病的各个方面，其定量分析的液相色谱-质谱（LC-MS）提出了实质性的技术挑战。作为脂质组学社区的成员，我们制定了技术建议，以确保LC-MS定量氧化脂质的最佳实践。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science Signaling BIOCHEMISTRY & MOLECULAR BIOLOGY-CELL BIOLOGY

CiteScore

9.50

自引率

0.00%

发文量

148

审稿时长

3-8 weeks

期刊介绍： "Science Signaling" is a reputable, peer-reviewed journal dedicated to the exploration of cell communication mechanisms, offering a comprehensive view of the intricate processes that govern cellular regulation. This journal, published weekly online by the American Association for the Advancement of Science (AAAS), is a go-to resource for the latest research in cell signaling and its various facets. The journal's scope encompasses a broad range of topics, including the study of signaling networks, synthetic biology, systems biology, and the application of these findings in drug discovery. It also delves into the computational and modeling aspects of regulatory pathways, providing insights into how cells communicate and respond to their environment. In addition to publishing full-length articles that report on groundbreaking research, "Science Signaling" also features reviews that synthesize current knowledge in the field, focus articles that highlight specific areas of interest, and editor-written highlights that draw attention to particularly significant studies. This mix of content ensures that the journal serves as a valuable resource for both researchers and professionals looking to stay abreast of the latest advancements in cell communication science.