{"title":"Comprehensive analysis of the transcriptome implicated in the immune response of Procambarus clarkii to Aeromonas hydrophila","authors":"Xi-Rong Zhu , Wen-Hui Tang , Qin Peng , Rui Zhang , Yao-Hui Chen , Qiu-Ning Liu , Bo-Ping Tang , Li-Shang Dai","doi":"10.1016/j.cbd.2025.101528","DOIUrl":null,"url":null,"abstract":"<div><div>In order to better understand the immune response of <em>Procambarus clarkii</em> to <em>Aeromonas hydrophila</em> injection, the transcriptome data of the gill tissue of <em>P. clarkii</em> were compared and analyzed. 1008 significant DEGs were identified in <em>A. hydrophila</em> infected and PBS control groups, including 411 up-regulated genes and 597 down-regulated genes. Endocytosis, phagocyte and lysosome were the most clustered pathways of DEGs in KEGG database. RNA-Seq results were validated through the verification of immune-related differentially expressed genes (DEGs), whose expression levels were assessed using quantitative real-time PCR (qRT-PCR). With the increase of treatment time of <em>A. hydrophila</em>, the total protein in gill of the treatment group showed a trend of increasing first, then decreasing and then increasing and decreasing. ACP and AKP both show a trend of rising first, then falling and then rising. The results of comprehensive research showed that crayfish infected with <em>A. hydrophila</em> caused damage to gill tissue, and the related immune genes were up-regulated and the immune mechanism was operated to protect the body from <em>A. hydrophila</em>. The differential gene <em>MRC1</em> was screened through the transcriptome, and to further understand its impact, it was disrupted by RNAi technology, which showed a significant down-regulation of immune genes (<em>TAB2</em>, <em>TLR3</em>, <em>ALF6</em>, <em>Lyso3</em>, <em>clotting factor G beta subunit-like</em> and <em>coagulation factor X-like</em>) as well as genes downstream of the pathway (<em>AP4E1</em>, <em>ARSB</em> and <em>TUBA1A</em>). This study provides a theoretical basis for further exploring the immune adaptability of aquatic animals under bacterial infection.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"56 ","pages":"Article 101528"},"PeriodicalIF":2.2000,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1744117X25001170","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In order to better understand the immune response of Procambarus clarkii to Aeromonas hydrophila injection, the transcriptome data of the gill tissue of P. clarkii were compared and analyzed. 1008 significant DEGs were identified in A. hydrophila infected and PBS control groups, including 411 up-regulated genes and 597 down-regulated genes. Endocytosis, phagocyte and lysosome were the most clustered pathways of DEGs in KEGG database. RNA-Seq results were validated through the verification of immune-related differentially expressed genes (DEGs), whose expression levels were assessed using quantitative real-time PCR (qRT-PCR). With the increase of treatment time of A. hydrophila, the total protein in gill of the treatment group showed a trend of increasing first, then decreasing and then increasing and decreasing. ACP and AKP both show a trend of rising first, then falling and then rising. The results of comprehensive research showed that crayfish infected with A. hydrophila caused damage to gill tissue, and the related immune genes were up-regulated and the immune mechanism was operated to protect the body from A. hydrophila. The differential gene MRC1 was screened through the transcriptome, and to further understand its impact, it was disrupted by RNAi technology, which showed a significant down-regulation of immune genes (TAB2, TLR3, ALF6, Lyso3, clotting factor G beta subunit-like and coagulation factor X-like) as well as genes downstream of the pathway (AP4E1, ARSB and TUBA1A). This study provides a theoretical basis for further exploring the immune adaptability of aquatic animals under bacterial infection.
期刊介绍:
Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology.
Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.