Salivary Testosterone, Androstenedione and 11-Oxygenated 19-Carbon Concentrations Differ by Age and Sex in Children.

IF 3 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Clinical Endocrinology Pub Date : 2025-05-20 DOI:10.1111/cen.15258

Julie Park, Andrew Titman, Orla Bright, Silothabo Dliso, Alena Shantsila, Gregory Y H Lip, Jo Adaway, Brian Keevil, Daniel B Hawcutt, Joanne Blair

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引用次数: 0

Abstract

Background: The diagnosis and management of childhood adrenal disorders is challenging. Clinical markers of hormone excess or deficiency may take months to manifest, and traditional biomarkers correlate only partially with clinical outcomes. Recent work has indicated that 11 oxygenated 19-carbon (11oxC19) steroids may be useful in the assessment of adrenal function. 11oxC19 steroids, testosterone (T) and androstenedione (A4), can be measured in saliva, but very little is known about these hormones in healthy children.

Methods: Participants collected saliva samples 30 min after waking and every 2 h until bedtime. Samples were analysed for T, A4, 11 ketotestosterone (11KT) and 11βhydroxyandrostenedione (11OHA4) by liquid chromatography tandem mass spectrometry.

Results: Fifty-two (30 male) healthy children aged 10.4 ± 3.9 (5.0-17.5) participated. Median height SDS was 0.4 (IQR -0.3 to 1.01) and median BMI SDS was 0.3 (IQR -0.2 to 1.3). All steroids showed a diurnal rhythm, with all hormones decreasing in measured concentration at time points that are 30 min after waking. Salivary T was higher in postpubertal children, particularly boys (p < 0.001). Salivary A4 was lower in boys compared to girls (p = 0.009) and did not differ with pubertal development. 11KT increased with age (p < 0.001) and concentrations were similar between boys and girls. 11OHA4 reduced in concentration with age (p = 0.03) and was below detectable limits after the early morning peak in both sexes.

Conclusion: For the first time we describe the physiological profile of 11KT and 11OHA4 in children. Further data are required to establish reference ranges, which should consider age, sex, pubertal status and time of sampling.

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儿童唾液睾酮、雄烯二酮和11-氧合19-碳浓度因年龄和性别而异。

背景：儿童肾上腺疾病的诊断和治疗具有挑战性。激素过量或缺乏的临床标志物可能需要数月才能显现出来，而传统的生物标志物仅与临床结果部分相关。最近的研究表明，11氧合19碳（11oxC19）类固醇可能有助于评估肾上腺功能。11oxC19类固醇，睾酮(T)和雄烯二酮（A4），可以在唾液中测量，但对健康儿童的这些激素知之甚少。方法：受试者在醒来后30分钟和就寝前每2小时采集一次唾液样本。采用液相色谱串联质谱法分析样品中T、A4、11酮睾酮（11KT）和11β羟基雄烯二酮（11OHA4）的含量。结果：参与调查的健康儿童52例（男30例），年龄10.4±3.9（5.0 ~ 17.5）岁。中位身高SDS为0.4 (IQR -0.3 ~ 1.01)，中位BMI SDS为0.3 （IQR -0.2 ~ 1.3）。所有类固醇均显示出昼夜节律，在醒来后30分钟的时间点，所有激素的测量浓度均下降。结论：本文首次描述了11KT和11OHA4在儿童中的生理特征。需要进一步的数据来建立参考范围，该范围应考虑年龄、性别、青春期状态和抽样时间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Endocrinology 医学-内分泌学与代谢

CiteScore

6.40

自引率

3.10%

发文量

192

审稿时长

1 months

期刊介绍： Clinical Endocrinology publishes papers and reviews which focus on the clinical aspects of endocrinology, including the clinical application of molecular endocrinology. It does not publish papers relating directly to diabetes care and clinical management. It features reviews, original papers, commentaries, correspondence and Clinical Questions. Clinical Endocrinology is essential reading not only for those engaged in endocrinological research but also for those involved primarily in clinical practice.